Anticorps Polyclonal de lapin anti-IFT57

• Phare
• Validé par KD/KO

IFT57 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

21

N° de cat : 11083-1-AP

Synonymes

DERP8, ESRRBL1, HIP1 interacting protein, HIPPI, IFT57, MHS4R2

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Galerie de données de validation

• 

  WB analysis of mouse retina using 11083-1-AP

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 11083-1-AP (IFT57 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11083-1-AP

  WB result of IFT57 antibody (11083-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IFT57 transfected HEK-293 cells.

• 

  WB analysis of HEK-293 using 11083-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11083-1-AP (IFT57 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11083-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11083-1-AP (IFT57 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11083-1-AP

  IP Result of anti-IFT57 (IP:11083-1-AP, 5ug; Detection:11083-1-AP 1:800) with HEK-293 cells lysate 2000ug.

• 

  IHC staining of human prostate cancer using 11083-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 11083-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 11083-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 11083-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 11083-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 11083-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 11083-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 11083-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 11083-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11083-1-AP (IFT57 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MDCK using 11083-1-AP

  Immunofluorescent analysis of MDCK cells using 11083-1-AP (IFT57 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rétinien de souris, cellules HEK-293
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MDCK,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below
• IF: See 11 publications below
• IP: See 2 publications below

Informations sur le produit

11083-1-AP cible IFT57 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFT57 Protéine recombinante Ag1566
• Nom complet: intraflagellar transport 57 homolog (Chlamydomonas)
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC011899
• Symbole du gène: IFT57
• Identification du gène (NCBI): 55081
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IFT57, also named as DERP8, ESRRBL1, HIPPI and MHS4R2, belongs to the IFT57 family. It is required for the formation of cilia. IFT57 plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. It is a component of IFT complex B which composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. In contrast to BBS proteins, IFT57 and IFT88 is found in almost every cilium.(PMID:22072986)

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.; Authors - Huijie Zhao
human	WB	Nat Cell Biol; Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.; Authors - Malavika Raman
mouse	WB	Dev Cell; The Intraflagellar Transport Protein IFT27 Promotes BBSome Exit from Cilia through the GTPase ARL6/BBS3.; Authors - Gerald M Liew
human	WB	Nat Commun; IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.; Authors - Nicolas Taulet
mouse	WB,IF	EMBO J; Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.; Authors - Shichao Duan
mouse	IF	Proc Natl Acad Sci U S A; Primary cilia signaling mediates intraocular pressure sensation.; Authors - Na Luo