Anticorps Polyclonal de lapin anti-IFT80

IFT80 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 25230-1-AP

Synonymes

ATD2, IFT80, KIAA1374, WDR56

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 25230-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25230-1-AP (IFT80 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 25230-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25230-1-AP (IFT80 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25230-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25230-1-AP (IFT80 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, tissu cérébral de souris, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

25230-1-AP cible IFT80 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IFT80 Protéine recombinante Ag18650
• Nom complet: intraflagellar transport 80 homolog (Chlamydomonas)
• Masse moléculaire calculée: 777 aa, 88 kDa
• Poids moléculaire observé: 88-90 kDa
• Numéro d’acquisition GenBank: BC101494
• Symbole du gène: IFT80
• Identification du gène (NCBI): 57560
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
	WB	EMBO Rep; Male infertility-associated Ccdc108 regulates multiciliogenesis via the intraflagellar transport machinery.; Authors - Huijie Zhao
mouse	WB	Mol Neurobiol; Inpp5e Regulated the Cilium-Related Genes Contributing to the Neural Tube Defects Under 5-Fluorouracil Exposure; Authors - Xiuwei Wang
rat	WB	Cell Physiol Biochem; iTRAQ-Based Proteomic Analysis of Neonatal Kidney from Offspring of Protein Restricted Rats Reveals Abnormalities in Intraflagellar Transport Proteins.; Authors - Xiaomei Liu
mouse	WB	Life (Basel); The Effect of IFT80 Deficiency in Osteocytes on Orthodontic Loading-Induced and Physiologic Bone Remodeling: In Vivo Study; Authors - Hyeran Helen Jeon
mouse	IF	Cell Insight; Endothelial RGS12 governs angiogenesis in inflammatory arthritis by controlling cilia formation and elongation via MYCBP2 signaling; Authors - Gongsheng Yuan

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