Anticorps Recombinant de lapin anti-IFT88

IFT88 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

230074E12

N° de cat : 82886-2-RR

Synonymes

TTC10, TG737, Intraflagellar transport protein 88 homolog, 230074E12

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Galerie de données de validation

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  WB analysis using 82886-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82886-2-RR (IFT88 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of hTERT-RPE1 using 82886-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using IFT88 antibody (82886-2-RR, Clone: 230074E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

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  FC experiment of HEK-293 using 82886-2-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug IFT88 Recombinant antibody (82886-2-RR, Clone:230074E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82886-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82886-2-RR against Human IFT88 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules PC-3
• Résultats positifs en IF/ICC: Starvation treated hTERT-RPE1 cells,
• Résultats positifs en FC (Intra): cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82886-2-RR cible IFT88 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IFT88 Protéine recombinante Ag4980
• Nom complet: intraflagellar transport 88 homolog (Chlamydomonas)
• Masse moléculaire calculée: 94 kDa
• Poids moléculaire observé: 88-95 kDa
• Numéro d’acquisition GenBank: BC030776
• Symbole du gène: IFT88
• Identification du gène (NCBI): 8100
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926).

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