Anticorps Polyclonal de lapin anti-IGBP1

• Phare
• Validé par KD/KO

IGBP1 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP,ELISA

Conjugaison

Non conjugué

N° de cat : 14952-1-AP

Synonymes

ALPHA 4, CD79a binding protein 1, IBP1, IGBP1, Protein alpha 4

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Galerie de données de validation

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  WB analysis of Jurkat using 14952-1-AP

  WB result of IGBP1 antibody (14952-1-AP, 1:1000) with si-control and si-IGBP1 transfected Jurkat cells.

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  WB analysis of Raji using 14952-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14952-1-AP (IGBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14952-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14952-1-AP (IGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14952-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14952-1-AP (IGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14952-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14952-1-AP (IGBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 14952-1-AP

  IP result of anti-IGBP1 (IP:14952-1-AP, 4ug; Detection:14952-1-AP 1:300) with Jurkat cells lysate 4000 ug.

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Applications testées

• Résultats positifs en WB: cellules Raji, Jurkat
• Résultats positifs en IP: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

14952-1-AP cible IGBP1 dans les applications de WB, IP,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IGBP1 Protéine recombinante Ag6782
• Nom complet: immunoglobulin (CD79A) binding protein 1
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC004137
• Symbole du gène: IGBP1
• Identification du gène (NCBI): 3476
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Immunoglobulin-binding protein 1 (IGBP1), also known as ALPHA-4, was originally identified as a signal transduction molecule coprecipitating with MB1 (CD79a) protein of the B-cell antigen receptor (BCR) complex. IGBP1 has been characterized as an essential regulator of the catalytic activity of protein phosphatase 2A (PP2A), which is a ubiquitous phosphatase with broad substrate specificity and diverse cellular functions.