Anticorps Polyclonal de lapin anti-IGF1R

• Phare
• Validé par KD/KO

IGF1R Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

45

Avis (1)

N° de cat : 20254-1-AP

Synonymes

CD221, IGF I receptor, IGF1R, IGF1R Beta Chain, IGFIR, IGFR, JTK13

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Galerie de données de validation

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  WB analysis of HeLa using 20254-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20254-1-AP (IGF1R beta chain antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20254-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20254-1-AP (IGF1R beta chain antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 20254-1-AP

  IP Result of anti-IGF1R beta chain (IP:20254-1-AP, 4ug; Detection:20254-1-AP 1:300) with HeLa cells lysate 2400ug.

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  IHC staining of human lung cancer using 20254-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20254-1-AP (IGF1R beta chain antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human lung cancer using 20254-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20254-1-AP (IGF1R beta chain antibody at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules L02
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 41 publications below
• IHC: See 7 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

20254-1-AP cible IGF1R dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: IGF I receptor
• Masse moléculaire calculée: 155 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: NM_000875
• Symbole du gène: IGF1R
• Identification du gène (NCBI): 3480
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cells. IGF1R is a heterotetrameric receptor, composed of two alpha subunits (130 kDa each) and two beta subunits (~98 kDa each) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds.

Publications

Species	Application	Title
human	WB	Gastroenterology; Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.; Authors - Haojie Jin
human	WB	Mol Cancer; CircVAPA promotes small cell lung cancer progression by modulating the miR-377-3p and miR-494-3p/IGF1R/AKT axis.; Authors - Jinghan Hua
mouse	WB	J Cachexia Sarcopenia Muscle; Exogenous insulin-like growth factor 1 attenuates cisplatin-induced muscle atrophy in mice.; Authors - Hiroyasu Sakai
human	WB	Clin Transl Med; Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.; Authors - An-Dong Liu
human	WB,IHC	Cancer Lett; Thevebioside, the active ingredient of traditional Chinese medicine, promotes ubiquitin-mediated SRC-3 degradation to induce NSCLC cells apoptosis.; Authors - Chao Yao
human	WB,IHC	Oncogene; Circulating IGF-1 promotes prostate adenocarcinoma via FOXO3A/BIM signaling in a double-transgenic mouse model.; Authors - Shuang Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This antibody had some faint non-specific bands in addition to a strong and specific IGF1R band.

• Applications: Western Blot
• Cell Tissue Type: Heart

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