Anticorps Monoclonal anti-IGF1R

IGF1R Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

3C6A11

N° de cat : 66283-1-Ig

Synonymes

CD221, IGF I receptor, IGF1R, IGF1R Beta Chain, IGF1R-Specific, IGFIR, IGF-IR beta, IGFR, JTK13

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Galerie de données de validation

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  WB analysis using 66283-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66283-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66283-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66283-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 66283-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66283-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66283-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 66283-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 66283-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66283-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66283-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A549, cellules C6, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HUVEC, cellules ROS1728
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de l'endomètre humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

66283-1-Ig cible IGF1R dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: IGF I receptor
• Masse moléculaire calculée: 155 kDa
• Poids moléculaire observé: 130-140 kDa
• Numéro d’acquisition GenBank: NM_000875
• Symbole du gène: IGF1R
• Identification du gène (NCBI): 3480
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IGF1R, also named CD221, belongs to the protein kinase superfamily, Tyr protein kinase family, and INS receptor subfamily. IGF1R binds INS-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, IGF1R binds IGF1. The hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2, and not significantly activated by INS, and that hybrid receptor composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2, and INS. In contrast, the hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for INS. Defects in IGF1R may be a cause in some cases of resistance to INS-like growth factor 1 (IGF1 resistance).

Publications

Species	Application	Title
human	IHC	Thyroid; A MULTICENTER, SINGLE-BLIND, CASE-CONTROL, IMMUNOHISTOCHEMICAL STUDY OF ORBITAL TISSUE IN THYROID EYE DISEASE; Authors - YuanPing Hai
human	WB	Cell Signal; Cell surface GRP78 facilitates hepatoma cells proliferation and migration by activating IGF-IR.; Authors - Yancun Yin
rat	WB	Behav Brain Res; Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats.; Authors - Shuai Lian
mouse	WB	Biosci Biotechnol Biochem; Black rice bran intake reduces phosphorylated tau levels and enhances insulin signaling in the brain of aged normal mice; Authors - Takashi Hara
human	WB	Eur J Obstet Gynecol Reprod Biol; Comprehensive assessment the expression of core elements related to IGFIR/PI3K pathway in granulosa cells of women with polycystic ovary syndrome.; Authors - Tingting He
human	WB	Drug Deliv; Codelivery of DOX and siRNA by folate-biotin-quaternized starch nanoparticles for promoting synergistic suppression of human lung cancer cells.; Authors - Liangping Li

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