Anticorps Monoclonal anti-IGF1R/CD221

IGF1R/CD221 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(7)

CloneNo.

3C6A11

N° de cat : 66283-1-Ig

Synonymes

IGF 1 receptor, IGF1R, IGF-I receptor, IGFIR, IGFR

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Galerie de données de validation

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  WB analysis using 66283-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66283-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66283-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66283-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 66283-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66283-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66283-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 66283-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 66283-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66283-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66283-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66283-1-Ig (IGF1R beta chain antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A549, cellules C6, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HUVEC, cellules ROS1728
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de l'endomètre humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

66283-1-Ig cible IGF1R/CD221 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: IGF I receptor
• Masse moléculaire calculée: 155 kDa
• Poids moléculaire observé: 130-140 kDa
• Numéro d’acquisition GenBank: NM_000875
• Symbole du gène: IGF1R
• Identification du gène (NCBI): 3480
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IGF1R, also named as CD221, is a receptor tyrosine kinase which mediates actions of INS-like growth factor 1 (IGF1). IGF1R binds IGF1 with high affinity and IGF2 and INS with a lower affinity, the activated IGF1R is involved in cell growth and survival control. It is crucial for tumor transformation and survival of malignant cells. IGF1R is translated as ~220 kDa pro-receptors (PMID: 33969359). IGF1R is a heterotetrameric receptor, composed of two alpha subunits (~130 kDa) and two beta subunits (~100-120 kDa) (PMID: 6279656). Both the alpha and beta subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds.

Publications

Species	Application	Title
human	IHC	Thyroid; A MULTICENTER, SINGLE-BLIND, CASE-CONTROL, IMMUNOHISTOCHEMICAL STUDY OF ORBITAL TISSUE IN THYROID EYE DISEASE; Authors - YuanPing Hai
human	WB	Cell Signal; Cell surface GRP78 facilitates hepatoma cells proliferation and migration by activating IGF-IR.; Authors - Yancun Yin
rat	WB	Behav Brain Res; Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats.; Authors - Shuai Lian
mouse	WB	Biosci Biotechnol Biochem; Black rice bran intake reduces phosphorylated tau levels and enhances insulin signaling in the brain of aged normal mice; Authors - Takashi Hara
Human		Oncol Rep; Macrophage renewal modes affect acquired resistance to gefitinib in EGFR‑mutant lung cancer PC‑9 cells; Authors - Baoxia Zhao
human	WB	Eur J Obstet Gynecol Reprod Biol; Comprehensive assessment the expression of core elements related to IGFIR/PI3K pathway in granulosa cells of women with polycystic ovary syndrome.; Authors - Tingting He

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