Anticorps Polyclonal de lapin anti-IGFBP1

IGFBP1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 13981-1-AP

Synonymes

AFBP, hIGFBP 1, IBP 1, IBP1, IGF binding protein 1, IGF BP25, IGFBP 1, IGFBP1, Placental protein 12, PP12

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Galerie de données de validation

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  WB analysis using 13981-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13981-1-AP (IGFBP1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 13981-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13981-1-AP (IGFBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, placenta humain
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 2 publications below

Informations sur le produit

13981-1-AP cible IGFBP1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IGFBP1 Protéine recombinante Ag5026
• Nom complet: IGF binding protein 1
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28-35 kDa
• Numéro d’acquisition GenBank: BC057806
• Symbole du gène: IGFBP1
• Identification du gène (NCBI): 3484
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IGF-binding proteins (IGFBPs) family contains members like IGFBP-1, -2, -3, -4,-5 and-6. IGFBPs prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. IGF-I physically interacts with IGFBP-1 and that IGFBP-1 also binds to an integrin receptor, but the effect of IGFBP-1 on cell migration may be independent of IGF-I and is probably mediated through the alpha 5 beta 1 integrin. Transgenic mice models demonstrated that IGFBPs played important roles in the pathogenesis of obesity and INS resistance. Studies conducted in humans demonstrated the close relation between IGFBPs and the components of the metabolic syndrome.

Publications

Species	Application	Title
human	WB	Clin Transl Med; Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.; Authors - Lijie Zhou
human	WB, IHC	Lung Cancer; Molecular profiling of thymoma with myasthenia gravis: Risk factors of developing myasthenia gravis in thymoma patients.; Authors - Ming-Ching Lee
human	WB	Phytomedicine; Effects of natural 24-epibrassinolide on inducing apoptosis and restricting metabolism in hepatocarcinoma cells; Authors - Hongfei Zhou
human	WB	Biol Reprod; IGFBP1 Involved in the Decreased Birth Weight Due to Fetal High Estrogen Exposure in Mice.; Authors - Min Jin
human	WB	Reprod Biol Endocrinol; Increased expression of prokineticin 2 and its receptor in endometrium of recurrent implantation failure patients decreased the expression of MMP9 important for decidualization.; Authors - Jun Zhai
human	WB	Reprod Biol Endocrinol; PMID: 29925391; Authors - Cheng Li

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