Anticorps Polyclonal de lapin anti-IGFBP2

IGFBP2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 15699-1-AP

Synonymes

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Galerie de données de validation

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  WB analysis of MCF-7 using 15699-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15699-1-AP (IGFBP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 15699-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15699-1-AP (IGFBP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 15699-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15699-1-AP (IGFBP2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15699-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15699-1-AP (IGFBP2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 15699-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 15699-1-AP (IGFBP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 15699-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 15699-1-AP (IGFBP2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 15699-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15699-1-AP (IGFBP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 15699-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15699-1-AP (IGFBP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 15699-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15699-1-AP (IGFBP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 15699-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15699-1-AP (IGFBP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15699-1-AP

  Immunofluorescent analysis of HeLa cells using 15699-1-AP (IGFBP2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules PC-3
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la prostate humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

15699-1-AP cible IGFBP2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IGFBP2 Protéine recombinante Ag8327
• Nom complet: IGF binding protein 2
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC004312
• Symbole du gène: IGFBP2
• Identification du gène (NCBI): 3485
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. IGFBP-2 is a 34 kD IGFBP protein with degarded fragments of 24 kD, 19kd and 15kd. The levels of IGFBP2 are elevated during progression of many human cancers. IGFBP2 is expressed in invasive PAc, whereas its expression in HG-PIN is low. It can be as an Immunohistochemical Marker for Prostatic Adenocarcinoma.