Anticorps Recombinant de lapin anti-IGFBP4

IGFBP4 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230543F8

N° de cat : 83200-3-RR

Synonymes

IBP 4, HT29 IGFBP, Deb2, BP4, BP 4

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Galerie de données de validation

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  WB analysis using 83200-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83200-3-RR (IGFBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83200-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83200-3-RR (IGFBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83200-3-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83200-3-RR (IGFBP4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of A549 using 83200-3-RR

  1x10^6 A549 cells were intracellularly stained with 0.4 ug IGFBP4 Recombinant antibody (83200-3-RR, Clone:230543F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83200-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83200-3-RR against Human IGFBP4 were performed. The affinity constant is 0.776 nM.

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-231, cellules K-562
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83200-3-RR cible IGFBP4 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: insulin-like growth factor binding protein 4
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28-40 kDa
• Numéro d’acquisition GenBank: NM_001552.3
• Symbole du gène: IGFBP4
• Identification du gène (NCBI): 3487
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IGFBP4, also named as IBP4, is a 258 amino acid protein, which contains one IGFBP N-terminal domain and one thyroglobulin type-1 domain. IGFBP4 is the smallest member of the insulin-like growth factor binding protein family (IGFBP). It is a hepatic protein that plays a role in modulating the activity and bioavailability of IGF-I. The expression of IGFBP4 was found to increase under conditions of hypoxia. IGFBP4 is highly expressed in adipocytes and osteoblasts and is inhibitory of IGFs in vitro. (PMID: 28938423, PMID: 34646401).

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