Anticorps Polyclonal de lapin anti-IGSF11

• Phare
• Validé par KD/KO

IGSF11 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 14003-1-AP

Synonymes

BT IgSF, BTIGSF, CXADRL1, IGSF11, Igsf13, VSIG3

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Galerie de données de validation

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  WB analysis of human brain using 14003-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14003-1-AP (IGSF11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 14003-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14003-1-AP (IGSF11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 14003-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14003-1-AP (IGSF11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14003-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14003-1-AP (IGSF11 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

14003-1-AP cible IGSF11 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IGSF11 Protéine recombinante Ag5094
• Nom complet: immunoglobulin superfamily, member 11
• Masse moléculaire calculée: 430 aa, 46 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: BC034411
• Symbole du gène: IGSF11
• Identification du gène (NCBI): 152404
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IHC,IF	Neurosci Lett; Promotion of differentiation in developing mouse cerebellar granule cells by a cell adhesion molecule BT-IgSF.; Authors - Kasumi Higashine ; KD Validated
human	IHC	Medicine (Baltimore); Prediction of lung squamous cell carcinoma immune microenvironment and immunotherapy efficiency with pyroptosis-derived genes; Authors - Xiaheng Deng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Dibyendu (Verified Customer) (04-30-2025)

I am writing regarding catalog number [A14003-1-AP] against [IGSF11]. Unfortunately, this antibody has proven unsuitable for our Western blot applications. In our hands it produces multiple non-specific bands across the entire cell lysate, and we are unable to distinguish any difference between knockdown and wild-type samples. As a result, we cannot reliably identify the target protein band, and the reagent has not yielded any usable data. Due to the promiscuity of the antibody we are even unsure of the results obtained from our IHC results using human cancer tissue samples. Could you please advise on a suitable alternative antibody clone or arrange for a refund? We appreciate your assistance in resolving this issue swiftly. Thank you for your prompt attention.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: 4T1

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