Anticorps Recombinant de lapin anti-IL-1 beta

IL-1 beta Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

241364B3

N° de cat : 98179-1-RR

Synonymes

IL 1beta, Il1b, IL-1,IL1, IL-1 beta,IL 1 beta,IL1beta, Il 1b

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Galerie de données de validation

• 

  FC experiment of mouse peritoneal macrophages using 98179-1-RR

  1x10^6 untreated or LPS and Brefeldin A treated mouse peritoneal macrophages were intracellularly stained with 0.25 ug Anti-Mouse IL-1 beta Rabbit Recombinant Antibody (98179-1-RR, Clone: 241364B3) and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC anti-Mouse CD11b. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98179-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98179-1-RR against Mouse IL-1 beta/IL-1F2 were performed. The affinity constant is 32.5 pM.

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Applications testées

• Résultats positifs en FC (Intra): LPS and Brefeldin A treated mouse peritoneal macrophages,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98179-1-RR cible IL-1 beta dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 1 beta
• Masse moléculaire calculée: 31kDa
• Numéro d’acquisition GenBank: NM_008361.4
• Symbole du gène: IL-1 beta
• Identification du gène (NCBI): 16176
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. Interleukin 1β(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions (PMID: 8630372; PMID: 12401481; PMID: 23704929; PMID: 24618930).

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