Anticorps Polyclonal de lapin anti-IL-10
IL-10 Polyclonal Antibody for WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain et plus (5)
Applications
WB, IHC, IF, ELISA
Conjugaison
Non conjugué
106
N° de cat : 20850-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | Protéine recombinante, ag14644 |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 70 publications below |
| IHC | See 25 publications below |
| IF | See 11 publications below |
| ELISA | See 1 publications below |
Informations sur le produit
20850-1-AP cible IL-10 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Réactivité citée | rat, bovin, Humain, porc, singe, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | IL-10 Protéine recombinante Ag14644 |
| Nom complet | interleukin 10 |
| Masse moléculaire calculée | 21 kDa |
| Numéro d’acquisition GenBank | BC104252 |
| Symbole du gène | IL10 |
| Identification du gène (NCBI) | 3586 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
The human IL10 gene is 4.7 kb in size and is located on the long arm of chromosome 1. It contains five exons. Biologically functional IL-10 exists in the form of a 36 kDa homodimer composed of two non-covalently bonded mono-mers each with 160 amino acids length. IL-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-10 antibody 20850-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun B cells sustain inflammation and predict response to immune checkpoint blockade in human melanoma. | ||
Microbiome Depletion of acetate-producing bacteria from the gut microbiota facilitates cognitive impairment through the gut-brain neural mechanism in diabetic mice. | ||
Acta Biomater A KPV-binding double-network hydrogel restores gut mucosal barrier in an inflamed colon. | ||
Acta Biomater Bio-inspired Glycosylated Nano-hydroxyapatites Enhance Endogenous Bone Regeneration by Modulating Macrophage M2 Polarization | ||
Int J Biol Macromol In situ polyphenol-adhesive hydrogel enhanced the noncarcinogenic repairing of KGF on the gut epithelial barrier on TNBS-induced colitis rats | ||
J Control Release In situ hydrogel capturing nitric oxide microbubbles accelerates the healing of diabetic foot |
