Anticorps Monoclonal anti-IL-12/IL-23 p40

IL-12/IL-23 p40 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

C17.8

N° de cat : 65097-1-Ig

Synonymes

Il12b, Il-12b, IL-12 p40, Il 12p40, Il 12b

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Galerie de données de validation

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  FC experiment of RAW 264.7 using 65097-1-Ig

  1X10^6 untreated or IFNγ, LPS, BFA and Monensin treated RAW 264.7 cells were intracellularly stained with 0.5 ug Anti-Mouse IL-12/IL-23 p40 (65097-1-Ig, Clone: C17.8) and FITC anti-rat IgG2a Antibody at dilution 1:100. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): IFNγ, LPS, BFA and Monensin treated RAW 264.7 cells

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

65097-1-Ig cible IL-12/IL-23 p40 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-12 p70 recombinante de souris exprimée par CHO
• Nom complet: interleukin 12b
• Numéro d’acquisition GenBank: BC103608
• Symbole du gène: Il12b
• Identification du gène (NCBI): 16160
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Interleukin (IL)-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

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