Anticorps Polyclonal de lapin anti-IL-12/IL-23 p40

• Phare
• Validé par KD/KO

IL-12/IL-23 p40 Polyclonal Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

N° de cat : 30956-1-AP

Synonymes

IL12B, CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL 12 P40, IL 12 subunit p40

---

Galerie de données de validation

• 

  WB analysis using 30956-1-AP

  Human IL-12B/p40, IL-12/p70, IL-12A/p35 recombinant protein, were subjected to SDS PAGE followed by western blot with 30956-1-AP (IL-12B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 30956-1-AP

  WB result of IL-12/IL-23 p40 antibody (30956-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-IL-12/IL-23 p40 transfected HeLa cells.

• 

  WB analysis using 30956-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30956-1-AP (IL-12B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of Jurkat using 30956-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug IL-12/IL-23 p40 Polyclonal antibody (30956-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of MOLT-4 using 30956-1-AP

  1x10^6 MOLT-4 cells were intracellularly stained with 0.4 ug IL-12/IL-23 p40 Polyclonal antibody (30956-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: Protéine recombinante, cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en FC (Intra): cellules Jurkat, cellules MOLT-4

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

30956-1-AP cible IL-12/IL-23 p40 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-12/IL-23 p40 Protéine recombinante Eg0431
• Nom complet: interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
• Masse moléculaire calculée: 328 aa, 37 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC067498
• Symbole du gène: IL12B
• Identification du gène (NCBI): 3593
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

{{ptg:RelatedPrimaryAntibodies}}