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Anticorps Monoclonal anti-IL-12RB1

IL-12RB1 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E10E10

N° de cat : CL488-66829

Synonymes

CD212, IL 12 receptor beta component, IL 12 receptor subunit beta 1, IL 12R beta 1, IL 12R BETA1, IL 12R subunit beta 1, IL 12RB1, IL12R, IL12RB, IL12RB1

Galerie de données de validation

Filter:
  • Flow cytometry (FC) experiment of K-562 cells using CoraLite® Plus 488-conjugated IL-12RB1 Monoclonal (CL488-66829)

    FC experiment of K-562 using CL488-66829

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IL-12RB1 (CL488-66829, Clone:1E10E10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en cytométriecellules K-562

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66829 cible IL-12RB1 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène IL-12RB1 Protéine recombinante Ag6920
Nom complet interleukin 12 receptor, beta 1
Masse moléculaire calculée 662 aa, 73 kDa
Poids moléculaire observé 100-110 kDa
Numéro d’acquisition GenBankBC029121
Symbole du gène IL12RB1
Identification du gène (NCBI) 3594
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL12RB1, also named as Interleukin-12 receptor subunit beta-1 or CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. Type 2 subfamily. IL12RB1 localizes in the membrane and functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.

Protocole

Product Specific Protocols
FC protocol for CL Plus 488 IL-12RB1 antibody CL488-66829Download protocol
Standard Protocols
Click here to view our Standard Protocols