Anticorps Recombinant de lapin anti-IL-17A

IL-17A Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

230038C7

N° de cat : 98005-1-RR

Synonymes

Il17a, IL-17A, interleukin 17A, Interleukin17A, 230038C7, IL 17A, Il17

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Galerie de données de validation

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  FC experiment of C57BL/6 Th17-polarized splenocytes using 98005-1-RR

  1x10^6 unstimulated or PMA and ionomycin stimulated (in the presence of protein transport inhibitors) Th17-polarized splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IL-17A (98005-1-RR, Clone: 230038C7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:700, and 0.06 ug CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98005-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98005-1-RR against Mouse IL-17A were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en FC (Intra): PMA and ionomycin stimulated Th17-polarized splenocytes
• Résultats positifs en cytométrie: PMA and ionomycin stimulated Th17-polarized splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98005-1-RR cible IL-17A dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 17A
• Masse moléculaire calculée: 17 kDa
• Numéro d’acquisition GenBank: NM-010552
• Symbole du gène: Il17a
• Identification du gène (NCBI): 16171
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

IL-17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.

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