Anticorps Recombinant de lapin anti-IL-17A

IL-17A Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

FC

Conjugaison

Non conjugué

CloneNo.

250413G7

N° de cat : 98564-1-RR

Synonymes

Il17a, Interleukin 17A

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Galerie de données de validation

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  FC experiment of Transfected HEK-293T using 98564-1-RR

  1x10^6 HEK-293T cells (left) and IL-17A transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Rat IL-17A Rabbit RecAb (98564-1-RR, Clone: 250413G7) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of Transfected HEK-293T using 98564-1-RR

  1x10^6 HEK-293Tcells (left) and IL-17A transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Rat IL-17A Rabbit RecAb (98564-1-RR, Clone: 250413G7) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en cytométrie: Transfected HEK-293T cells,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98564-1-RR cible IL-17A dans les applications de FC et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-17A Protéine recombinante Eg3339
• Nom complet: similar to Interleukin-17 precursor (IL-17) (Cytotoxic T lymphocyte-associated antigen 8) (CTLA-8)
• Masse moléculaire calculée: 17kDa
• Symbole du gène: LOC301289
• Identification du gène (NCBI): 301289
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

IL-17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.

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