Anticorps Recombinant de lapin anti-IL-17F

IL-17F Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230026G6

N° de cat : CL488-98019

Synonymes

Il17f, interleukin 17F, IL 17F, C87042, 230026G6

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Galerie de données de validation

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  FC experiment of C57BL/6 Th17-polarized splenocytes using CL488-98019

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated C57BL/6 Th17-polarized splenocytes were intracellularly stained with 0.25ug CoraLite® Plus 488 Anti-Mouse IL-17F Rabbit Recombinant Antibody (CL488-98019, Clone:230026G6). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): PMA, Ionomycin and Brefeldin A treated C57BL/6 Th17-polarized splenocytes,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-98019 cible IL-17F dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 17F
• Masse moléculaire calculée: 17KD
• Numéro d’acquisition GenBank: NM_145856.2
• Symbole du gène: IL-17F
• Identification du gène (NCBI): 257630
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

The interleukin 17 (IL-17) family of cytokines contains 6 structurally related cytokines, IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. IL-17 family plays crucial roles in host defense against microbial organisms and in the development of inflammatory diseases. IL-17A is a pro-inflammatory cytokine that also has the capacity to promote angiogenesis and osteoclastogenesis. IL-17F shares the highest homology with IL-17A and signals via a receptor composed by the IL-17RA and IL-17RC subunits. IL-17A and IL-17F can form IL-17A/A or IL-17F/F homodimers, IL-17A/F heterodimers are also formed. IL-17A and IL-17F, produced by the Th17 CD4(+) T cell lineage, have been linked to a variety of inflammatory and autoimmune conditions. IL-17F levels are elevated in sera and lesional psoriatic skin compared to non-lesional tissue. IL-17F also has been implicated in the development of neutrophilic airway inflammation.

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