Anticorps Polyclonal de lapin anti-IL-1R1

IL-1R1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 27348-1-AP

Synonymes

IL1R1, IL 1R alpha, IL 1R 1, EC:3.2.2.6, D2S1473

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Galerie de données de validation

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  WB analysis using 27348-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27348-1-AP (IL-1R1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 27348-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27348-1-AP (IL-1R1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 27348-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27348-1-AP (IL-1R1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 27348-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 27348-1-AP (IL-1R1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 27348-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 27348-1-AP (IL-1R1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules MOLT-4, cellules NIH/3T3, cellules Raji, tissu hépatique de souris
• Résultats positifs en IHC: tissu splénique de souris, tissu d'appendicite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

27348-1-AP cible IL-1R1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-1R1 Protéine recombinante Ag25998
• Nom complet: interleukin 1 receptor, type I
• Masse moléculaire calculée: 569 aa, 65 kDa
• Poids moléculaire observé: 80 kDa, 60 kDa
• Numéro d’acquisition GenBank: BC075063
• Symbole du gène: IL1R1
• Identification du gène (NCBI): 3554
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL-1R1 (IL-1 receptor type 1, also known as p80, IL-1R-alpha or CD121a) is a 80 kDa transmembrane glycoprotein and a member of the interleukin-1 receptor family (PMID: 35163653; 29248002). It is a receptor for IL-1α, IL-1β, and interleukin-1 receptor antagonist (IL-1Ra). IL-1α and IL-1β interact with the extracellular domain of IL-1R1, triggering the recruitment of an accessory receptor, the IL-1RAcP, resulting in a functional receptor complex that initiates IL-1R1 signaling cascades (PMID: 35163653). IL-1Ra competes with active IL-1 and blocks binding to their common activating receptor IL-1R1 and, thus, inhibits IL-1 signaling (PMID: 35332327; 11641511). It has been reported that IL-1R1 may also bind IL-38 (PMID: 29248002). IL-1R1 expression has been found in many cell types including T cells, macrophages, neutrophils, eosinophils, basophils, mast cells, fibroblasts, and endothelial cells. IL-1R1 can be released from the cell surface following proteolytic cleavage, generating a soluble form of 55-60 kDa (PMID: 17324958).

Publications

Species	Application	Title
mouse	IHC	Exp Gerontol; Endoplasmic reticulum stress-induced necroptosis promotes cochlear inflammation: Implications for age-related hearing loss; Authors - Zhongwu Su
mouse	IF	J Hazard Mater; Potential mechanisms of aortic medial degeneration promoted by co-exposure to microplastics and lead; Authors - Xiaoping Xie
mouse	IF	Front Neurosci; Oxidative stress and inflammation combine to exacerbate cochlear damage and sensorineural hearing loss in C57BL/6 mice; Authors - Zhongwu Su

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