Anticorps Recombinant de lapin anti-IL-2

IL-2 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

240358A2

N° de cat : 98055-1-RR

Synonymes

Il2, T-cell growth factor, Interleukin-2, interleukin 2, Il 2

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Galerie de données de validation

• 

  FC experiment of mouse splenocytes using 98055-1-RR

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated mouse splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IL-2 Rabbit Recombinant Antibody (98055-1-RR, Clone: 240358A2) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98055-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98055-1-RR against Mouse IL-2 were performed. The affinity constant is 0.229 nM.

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Applications testées

• Résultats positifs en FC (Intra): PMA, Ionomycin and Brefeldin A treated mouse splenocytes,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98055-1-RR cible IL-2 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 2
• Masse moléculaire calculée: 19 kDa
• Numéro d’acquisition GenBank: NM_008366
• Symbole du gène: IL-2
• Identification du gène (NCBI): 16183
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of the IL-2Rβ and IL-2Rγ receptor subunits (PMID: 25992858, 24907378, 15034008, 17981641).

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