Anticorps Recombinant de lapin anti-IL-2

IL-2 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

240358A2

N° de cat : CL594-98055

Synonymes

Il2, 240358A2, Il 2, interleukin 2, Interleukin-2

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Galerie de données de validation

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  FC experiment of mouse splenocytes using CL594-98055

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated mouse splenocytes were intracellularly stained with CoraLite® Plus 488 Anti-Mouse CD4 and 0.25 ug CoraLite®594 Anti-Mouse IL-2 Rabbit RecAb (CL594-98055, Clone: 240358A2). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of mouse splenocytes using CL594-98055

  1x10^6 PMA, Ionomycin and Brefeldin A treated mouse splenocytes were intracellularly stained with 0.25 ug CoraLite®594 Anti-Mouse IL-2 Rabbit RecAb (CL594-98055, Clone: 240358A2) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). 1x10^6 untreated mouse splenocytes were intracellularly stained with 0.25 ug CoraLite®594 Anti-Mouse IL-2 Rabbit RecAb (CL594-98055, Clone: 240358A2) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): PMA, Ionomycin and Brefeldin A treated mouse splenocytes,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-98055 cible IL-2 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-2 Protéine recombinante Eg0762
• Nom complet: interleukin 2
• Masse moléculaire calculée: 19 kDa
• Numéro d’acquisition GenBank: NM_008366
• Symbole du gène: IL-2
• Identification du gène (NCBI): 16183
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of the IL-2Rβ and IL-2Rγ receptor subunits (PMID: 25992858, 24907378, 15034008, 17981641).

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