Anticorps Recombinant de lapin anti-IL-2

IL-2 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

240416E3

N° de cat : FITC-98053

Synonymes

IL2, 240416E3, IL 2, interleukin 2, Interleukin-2

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Galerie de données de validation

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  FC experiment of human PBMCs using FITC-98053

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug FITC Plus Anti-Human IL-2 Rabbit RecAb (FITC-98053, Clone: 240416E3) and APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): PMA, Ionomycin and Brefeldin A treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-98053 cible IL-2 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-2 Protéine recombinante Eg0828
• Nom complet: interleukin 2
• Masse moléculaire calculée: 153 aa, 18 kDa
• Numéro d’acquisition GenBank: BC070338
• Symbole du gène: IL2
• Identification du gène (NCBI): 3558
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of the IL-2Rβ and IL-2Rγ receptor subunits (PMID: 25992858, 24907378, 15034008, 17981641).

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