Anticorps Recombinant de lapin anti-IL-21

IL-21 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

230267G7

N° de cat : 98015-1-RR

Synonymes

Il21, interleukin 21, 230267G7

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Galerie de données de validation

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  FC experiment of C57BL/6 Th17-polarized splenocytes using 98015-1-RR

  1x10^6 untreated or PMA, Ionomycin, and Brefeldin A treated C57BL/6 Th17-polarized splenocytes were intracellularly stained with APC Anti-Mouse CD4 (GK1.5) and 0.25 ug Anti-Mouse IL-21 Rabbit Recombinant Antibody (98015-1-RR, Clone: 230267G7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L).Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98015-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98015-1-RR against Mouse IL-21 were performed. The affinity constant is 1.29 nM.

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Applications testées

• Résultats positifs en FC (Intra): PMA, Ionomycin and Brefeldin A treated mouse splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98015-1-RR cible IL-21 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 21
• Masse moléculaire calculée: 17 aa
• Numéro d’acquisition GenBank: NM_001291041
• Symbole du gène: IL-21
• Identification du gène (NCBI): 60505
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Interleukin 21 (IL-21) is a cytokine produced predominantly by cluster of differentiation 4 (CD4+) T-cells and natural killer T-cells. IL-21 is preliminarily expressed in activated cluster of differentiation 4 (CD4+) T-cells and natural killer (NK) T-cells. IL-21 receptor (IL-21R) is a class I cytokine receptor, which requires dimerization with the indispensable common gamma chain (γc) subunit to bind IL-21. IL-21 plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. IL-21 mediates apoptosis in B-CLL cells through up-regulation of the BIM (BH3 family member) and enhances both direct and antibody-dependent cellular cytotoxicity in these cells.

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