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Anticorps Monoclonal anti-NeutraKine® IL-12/IL-23 p40

NeutraKine® IL-12/IL-23 p40 Monoclonal Antibody for Neutralization, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

Neutralization, ELISA

Conjugaison

Non conjugué

CloneNo.

2A9H6

N° de cat : 69006-1-Ig

Synonymes

Cytotoxic lymphocyte maturation factor 40 kDa subunit (CLMF p40), IL 23, IL-12 subunit p40, IL-12/IL-23 p40, IL12B, IL-12B, IL-23 p40, IL23A, IL-23B, IL23P19, NeutraKine® IL-23 p40, SGRF

Galerie de données de validation

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  • Neutralization experiment of NeutraKine® IL-23 p40 using NeutraKine® IL-23 p40 Monoclonal antibody (69006-1-Ig)

    Neutralization experiment of NeutraKine® IL-23 p40 using 69006-1-Ig

    Recombinant human IL-23 (Cat.NO. HZ-1254) induces invitro cultured mouse splenocytes to secret IL-17A in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human IL-23 (4 ng/mL HZ-1254) is neutralized by mouse anti-human IL-23 p40 monoclonal antibody 69006-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 8-30 ng/mL.

  • Neutralization experiment of NeutraKine® IL-23 p40 using NeutraKine® IL-23 p40 Monoclonal antibody (69006-1-Ig)

    Neutralization experiment of NeutraKine® IL-23 p40 using 69006-1-Ig

    Recombinant human IL-23 (Cat.NO. HZ-1254) induces in vitro cultured mouse splenocytes to secret IL-17A in a dose-dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human IL-23 (4 ng/mL HZ-1254) is neutralized by mouse anti-human IL-23 p40 monoclonal antibody 69006-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 8-30 ng/mL. The NeutraControl mouse anti-human IL-23 p40 monoclonal antibody 69506-1-Ig could recognize human IL-23, but could not neutralize human IL-23 in the range of 0.244-4000ng/mL(green curve, refer to top X-right Y axis).

  • ELISA experiment of Recombinant protein using NeutraKine® IL-23 p40 Monoclonal antibody (69006-1-Ig)

    ELISA experiment of Recombinant protein using 69006-1-Ig

    Indirect ELISA was carried out by coating recombinant Human IL-23 (Cat.NO. HZ-1254) at 70 ng/well followed by blocking and adding serial diluted IL-23 antibody 69006-1-Ig and 69506-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

  • ELISA experiment of Recombinant protein using NeutraKine® IL-12/IL-23 p40 Monoclonal antibody (69006-1-Ig)

    ELISA experiment of Recombinant protein using 69006-1-Ig

    Indirect ELISA was carried out by coating recombinant Human IL-12 (Cat.NO. HZ-1256) and IL-12 p40 (Cat.NO. HZ-1321) respectively at 70 ng/well followed by blocking and adding serial diluted 69029-1-Ig, 69529-1-Ig and 69006-1-Ig respectively. HRP-goat anti-mouse was used for detection. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. The result suggests that 69029-1-Ig and 69529-1-Ig only recognize IL-12(p35 and p40 heterodimer) but not recognize IL-12/IL-23 p40.

Neutralization

Informations sur le produit

69006-1-Ig cible NeutraKine® IL-12/IL-23 p40 dans les applications de Neutralization, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène human Humankine IL-12/IL-23 p40 protein HZ-1254
Nom complet interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
Masse moléculaire calculée 328 aa, 37 kDa
Numéro d’acquisition GenBankBC067498
Symbole du gène IL12B
Identification du gène (NCBI) 51561,3593
Conjugaison Non conjugué
Forme Lyophilized Powder
Méthode de purification Purification par protéine G
Tampon de stockage PBS stérile.
Endotoxin<0.1 EU/μg
ReconstitutionThis product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
Stability and StorageLyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Informations générales

IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

This antibody can be used to neutralize the bioactivity of IL-12/IL-23 p40.