Anticorps Recombinant de lapin anti-IL-4

IL-4 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

Biotin

CloneNo.

240794E11

N° de cat : Biotin-FcA98138

Synonymes

IL4, B-cell stimulatory factor 1, BSF-1, IL 4, interleukin 4

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Galerie de données de validation

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  FC experiment of human PBMCs using Biotin-FcA98138

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Biotin Anti-Human IL-4 Rabbit RecAb (Biotin-FcA98138, Clone: 240794E11) and PE-conjugated streptavidin. Cells were co-stained with FcZero-rAb™ APC Anti-Human CD3 (OKT3) Rabbit IgG Recombinant Antibody (APC-FcA65569, Clone: OKT3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): PMA, Ionomycin and Brefeldin A treated human PBMCs

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

Biotin-FcA98138 cible IL-4 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-4 Protéine recombinante Eg0104
• Nom complet: interleukin 4
• Masse moléculaire calculée: 153 aa, 17 kDa
• Numéro d’acquisition GenBank: BC070123
• Symbole du gène: IL-4
• Identification du gène (NCBI): 3565
• Conjugaison: Biotin
• Excitation/Emission maxima wavelengths: -
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

IL-4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.

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