Anticorps Recombinant de lapin anti-IL-4

IL-4 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

240794E11

N° de cat : CL594-98138

Synonymes

IL4, 240794E11, B-cell stimulatory factor 1, BSF-1, IL 4

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Galerie de données de validation

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  FC experiment of human PBMCs using CL594-98138

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 5 ul CoraLite®594 Anti-Human IL-4 Rabbit RecAb (CL594-98138, Clone: 240794E11). Cells were co-stained with CoraLite® Plus 488 Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody (CL488-65570, Clone: UCHT1). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): PMA, Ionomycin and Brefeldin A treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-98138 cible IL-4 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-4 Protéine recombinante Eg0104
• Nom complet: interleukin 4
• Masse moléculaire calculée: 153 aa, 17 kDa
• Numéro d’acquisition GenBank: BC070123
• Symbole du gène: IL-4
• Identification du gène (NCBI): 3565
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

IL-4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.

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