Anticorps Recombinant de lapin anti-IL-4

IL-4 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

241249H7

N° de cat : CL647-98255

Synonymes

241249H7, B-cell growth factor 1, B-cell IGG differentiation factor, B-cell stimulatory factor 1, BSF-1

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Galerie de données de validation

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  FC experiment of Transfected HEK-293T using CL647-98255

  1x10^6 untransfected or IL-4 transfected HEK-293T cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Rat IL-4 Rabbit RecAb (CL647-98255, Clone: 241249H7). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293T using CL647-98255

  1x10^6 untransfected or IL-4 transfected HEK-293T cells were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Rat IL-4 Rabbit RecAb (CL647-98255, Clone: 241249H7) (red) or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en FC (Intra): Transfected HEK-293T cells,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-98255 cible IL-4 dans les applications de FC (Intra) et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-4 Protéine recombinante Eg1113
• Nom complet: interleukin 4
• Masse moléculaire calculée: 16kDa
• Numéro d’acquisition GenBank: NM_201270.1
• Symbole du gène: IL-4
• Identification du gène (NCBI): 287287
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

IL-4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.

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