Anticorps Recombinant de lapin anti-IL-5

IL-5 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

240915D12

N° de cat : 98178-1-RR

Synonymes

IL5, Interleukin-5, Interleukin 5, IL 5, B-cell differentiation factor I

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Galerie de données de validation

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  FC experiment of human PBMCs using 98178-1-RR

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-5 Rabbit Recombinant Antibody (98178-1-RR, Clone: 240915D12) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98178-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98178-1-RR against Human IL-5 were performed. The affinity constant is 0.689 nM.

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Applications testées

• Résultats positifs en FC (Intra): PMA, Ionomycin and Brefeldin A treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98178-1-RR cible IL-5 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 5 (colony-stimulating factor, eosinophil)
• Masse moléculaire calculée: 134 aa, 15 kDa
• Numéro d’acquisition GenBank: BC066282
• Symbole du gène: IL5
• Identification du gène (NCBI): 3567
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

IL-5 is a 4-α-helix, pleiotropic cytokine that regulates the activities of B cells, eosinophils, and basophils. It is produced mostly by CD4+ T cells, eosinophils, and basophils, and signals through its specific receptor (IL-5R) on target cells to activate downstream JAK/STAT, Btk, and Ras/ERk pathways. Due to its pleiotropic effects, IL-5 plays a role in both innate and adaptive immune responses. It is a strong promoter of eosinophil differentiation, migration, maturation, and survival. Additionally, IL-5 is also capable of stimulating activated B cells to differentiate into antibody-secreting cells. Elevated IL-5 levels are strongly correlated with eosinophilia, which is a hallmark feature of allergy, asthma, and hypersensitivity. (PMID: 21986312, 22174445, 19819937, 16895557)

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