Anticorps Recombinant de lapin anti-IL-6

IL-6 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC (Intra)

Conjugaison

Non conjugué

CloneNo.

240701E8

N° de cat : 98089-1-RR

Synonymes

Il6, interleukin-6, interleukin 6, Il 6, 240701E8

---

Galerie de données de validation

• 

  FC experiment of RAW 264.7 using 98089-1-RR

  1x10^6 LPS treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse IL-6 Rabbit Recombinant Antibody (98089-1-RR, Clone:240701E8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L),or 1x10^6 untreated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse IL-6 Rabbit Recombinant Antibody (98089-1-RR, Clone:240701E8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of RAW 264.7 using 98089-1-RR

  1x10^6 LPS treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse IL-6 Rabbit Recombinant Antibody (98089-1-RR, Clone:240701E8) (red), or Isotype Control (blue), or 1x10^6 LPS treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse IL-6 Rabbit Recombinant Antibody (98089-1-RR, Clone:240701E8) (green)and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 98089-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98089-1-RR against Mouse IL-6 were performed. The affinity constant is 0.795 nM.

---

Applications testées

• Résultats positifs en FC (Intra): cellules RAW 264.7 traitées au LPS,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98089-1-RR cible IL-6 dans les applications de FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 6
• Masse moléculaire calculée: 24 kDa
• Numéro d’acquisition GenBank: NM_031168
• Symbole du gène: Il6
• Identification du gène (NCBI): 16193
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

{{ptg:RelatedPrimaryAntibodies}}