Anticorps Recombinant de lapin anti-IL-6

IL-6 Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

240670D12

N° de cat : CL594-98087

Synonymes

IL6, 240670D12, B-cell stimulatory factor 2, IFN-beta-2, IL 6

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Galerie de données de validation

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  FC experiment of human PBMCs using CL594-98087

  1x10^6 untreated or LPS and Brefeldin A treated human PBMCs were intracellularly stained with 5 ul CoraLite®594 Anti-Human IL-6 Rabbit RecAb (CL594-98087, Clone:240670D12). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

• 

  FC experiment of human PBMCs using CL594-98087

  1x10^6 LPS and Brefeldin A treated human PBMCs were intracellularly stained with 5 ul CoraLite®594 Anti-Human IL-6 Rabbit RecAb (CL594-98087, Clone: 240670D12) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were intracellularly stained with 5 ul CoraLite®594 Anti-Human IL-6 Rabbit RecAb (CL594-98087, Clone: 240670D12) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

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Applications testées

• Résultats positifs en FC (Intra): LPS and Brefeldin A treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-98087 cible IL-6 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 6 (interferon, beta 2)
• Masse moléculaire calculée: 212 aa, 24 kDa
• Numéro d’acquisition GenBank: BC015511
• Symbole du gène: IL6
• Identification du gène (NCBI): 3569
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

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