Anticorps Monoclonal anti-IL-6

IL-6 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4G3E6

N° de cat : CL647-69001

Synonymes

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFN beta 2, IFNB2, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6

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Galerie de données de validation

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  FC experiment of HUVEC using CL647-69001

  1X10^6 HUVEC cells untreated (dashed lines) or treated with LPS and Brefeldin A (red) were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Human IL-6 (CL647-69001, Clone:4G3E6), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HT-1080 using CL647-69001

  1X10^6 HT-1080 cells were treated with fixation and permeabilization reagents and intracellularly stained with 0.5 ug CoraLite® Plus 647 Anti-Human IL-6 (CL647-69001, Clone:4G3E6) (blue), and 0.5 ug Control Antibody.

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Applications testées

• Résultats positifs en cytométrie: cellules HUVEC traitées au LPS et à la bréfeldine A, cellules HUVEC

Dilution recommandée

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-69001 cible IL-6 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-6 Protéine recombinante HZ-1019
• Nom complet: interleukin 6 (interferon, beta 2)
• Symbole du gène: IL6
• Identification du gène (NCBI): 3569
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

This antibody is used to Block/Neutralize IL6 protein.

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C