- Phare
- Validé par KD/KO
Anticorps Monoclonal anti-IL-1 beta
IL-1 beta Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain et plus (2)
Applications
WB, IHC, IF/ICC, IP, ELISA, Cell treatment
Conjugaison
Non conjugué
CloneNo.
2A1B4
N° de cat : 66737-1-Ig
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules THP-1, cellules A431, cellules MG U-87 | 
| Résultats positifs en IHC | tissu de cancer du poumon humain,  tissu de cancer de la peau humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. | 
| Résultats positifs en IF/ICC | cellules THP-1 traitées au LPS, | 
Dilution recommandée
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 | 
| Immunohistochimie (IHC) | IHC : 1:50-1:500 | 
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 1 publications below | 
| WB | See 83 publications below | 
| IHC | See 13 publications below | 
| IF | See 11 publications below | 
| IP | See 1 publications below | 
| ELISA | See 2 publications below | 
Informations sur le produit
66737-1-Ig cible IL-1 beta dans les applications de WB, IHC, IF/ICC, IP, ELISA, Cell treatment et montre une réactivité avec des échantillons Humain
| Réactivité | Humain | 
| Réactivité citée | bovin, Humain, Lapin | 
| Hôte / Isotype | Mouse / IgG1 | 
| Clonalité | Monoclonal | 
| Type | Anticorps | 
| Immunogène | IL-1 beta Protéine recombinante Ag26030 | 
| Nom complet | interleukin 1, beta | 
| Masse moléculaire calculée | 269 aa, 31 kDa | 
| Poids moléculaire observé | 35 kDa | 
| Numéro d’acquisition GenBank | BC008678 | 
| Symbole du gène | IL1B | 
| Identification du gène (NCBI) | 3553 | 
| Conjugaison | Non conjugué | 
| Forme | Liquide | 
| Méthode de purification | Purification par protéine G | 
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol | 
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. | 
Informations générales
Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-1 beta antibody 66737-1-Ig | Download protocol | 
| IHC protocol for IL-1 beta antibody 66737-1-Ig | Download protocol | 
| IF protocol for IL-1 beta antibody 66737-1-Ig | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
Publications
| Species | Application | Title | 
|---|---|---|
| Cell Metab Lighting up arginine metabolism reveals its functional diversity in physiology and pathology | ||
| Adv Mater Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment | ||
| Biomaterials Macromechanics and polycaprolactone fiber organization drive macrophage polarization and regulate inflammatory activation of tendon in vitro and in vivo. | ||
| J Nanobiotechnology Low-dose celecoxib-loaded PCL fibers reverse intervertebral disc degeneration by up-regulating CHSY3 expression | ||
| J Nanobiotechnology Implantation of biomimetic polydopamine nanocomposite scaffold promotes optic nerve regeneration through modulating inhibitory microenvironment | ||
| Cell Mol Life Sci circACTA2 inhibits NLRP3 inflammasome-mediated inflammation via interacting with NF-κB in vascular smooth muscle cells | 











