Anticorps Monoclonal anti-IL-1 beta

• Phare
• Validé par KD/KO

IL-1 beta Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, IP, ELISA, Cell treatment

Conjugaison

Non conjugué

Publications(102)

CloneNo.

2A1B4

N° de cat : 66737-1-Ig

Synonymes

2A1B4, Catabolin, IL 1, IL 1 beta, IL1 beta

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Galerie de données de validation

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  WB analysis using 66737-1-Ig

  Untreated, LPS and Brefeldin A-treated THP-1 cells were subjected to SDS PAGE followed by western blot with 66737-1-Ig (IL-1 beta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66737-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66737-1-Ig (IL-1 Beta antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-87 MG using 66737-1-Ig

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 66737-1-Ig (IL-1 Beta antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 66737-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66737-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 66737-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 66737-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 66737-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 66737-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66737-1-Ig (IL1 beta antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of THP-1 using 66737-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed LPS treated THP-1 cells using IL-1 beta antibody (66737-1-Ig, Clone: 2A1B4 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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  Affinity and Kinetic Characterization of 66737-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 66737-1-Ig against Human IL1B were performed. The affinity constant is 5.03 nM.

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules A431, cellules MG U-87
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer de la peau humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules THP-1 traitées au LPS,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 83 publications below
• IHC: See 13 publications below
• IF: See 11 publications below
• IP: See 1 publications below
• ELISA: See 2 publications below

Informations sur le produit

66737-1-Ig cible IL-1 beta dans les applications de WB, IHC, IF/ICC, IP, ELISA, Cell treatment et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: bovin, Humain, Lapin
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-1 beta Protéine recombinante Ag26030
• Nom complet: interleukin 1, beta
• Masse moléculaire calculée: 269 aa, 31 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC008678
• Symbole du gène: IL1B
• Identification du gène (NCBI): 3553
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.

Publications

Species	Application	Title
human	WB	Cell Metab; Lighting up arginine metabolism reveals its functional diversity in physiology and pathology; Authors - Rui Li
	IF	Adv Mater; Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment; Authors - Yuewen Zhai
human	IF	Biomaterials; Macromechanics and polycaprolactone fiber organization drive macrophage polarization and regulate inflammatory activation of tendon in vitro and in vivo.; Authors - Angelina D Schoenenberger
	IHC	J Nanobiotechnology; Low-dose celecoxib-loaded PCL fibers reverse intervertebral disc degeneration by up-regulating CHSY3 expression; Authors - Yunhao Wang
	WB,IF	J Nanobiotechnology; Implantation of biomimetic polydopamine nanocomposite scaffold promotes optic nerve regeneration through modulating inhibitory microenvironment; Authors - Tonghe Pan
	WB,IHC	Cell Mol Life Sci; circACTA2 inhibits NLRP3 inflammasome-mediated inflammation via interacting with NF-κB in vascular smooth muscle cells; Authors - Yang Bai

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