Anticorps Polyclonal de lapin anti-IL-10RB

IL-10RB Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 15102-1-AP

Synonymes

CDW210B, CRF2 4, CRFB4, D21S58, D21S66, IL 10 receptor subunit beta, IL 10R subunit 2, IL 10R subunit beta, IL 10R2, IL 10RB, IL10RB, IL-10RB, IL-10Rβ, interleukin 10 receptor, beta

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Galerie de données de validation

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  WB analysis of rat skeletal muscle using 15102-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15102-1-AP (IL10RB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15102-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15102-1-AP (IL10RB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15102-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15102-1-AP (IL10RB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de rat, cellules Jurkat, tissu de muscle squelettique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

15102-1-AP cible IL-10RB dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-10RB Protéine recombinante Ag7445
• Nom complet: interleukin 10 receptor, beta
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 42-45 kDa
• Numéro d’acquisition GenBank: BC001903
• Symbole du gène: IL10RB
• Identification du gène (NCBI): 3588
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-10 (IL-10) is an anti-inflammatory cytokine that plays a critical role in maintaining immune system balance. IL-10 signals through a receptor complex consisting of two molecules of the IL-10R alpha-chain (IL-10RA) and two molecules of the accessory IL-10R beta-chain (IL-10RB) (PMID: 22236434). IL-10RA and IL-10RB are members of the type II cytokine receptor family. IL-10RB, also known as IL-10R2 or CDw210b, is the signaling subunit of the IL-10R complex and is constitutively expressed in a variety of cell types (PMID: 24507158). Binding of IL-10 to the IL-10R complex leads to JAK1- and TYK2-mediated phosphorylation of STAT3 (PMID: 11244051; 10433356). IL-10RB is also shared by several other cytokines, including IL22, IL-26, IFN-γ, IL-28A/B and IL29 (PMID: 22236434).

Publications

Species	Application	Title
human	WB	Biochim Biophys Acta Mol Basis Dis; IL-22 ameliorated cardiomyocyte apoptosis in cardiac ischemia/reperfusion injury by blocking mitochondrial membrane potential decrease, inhibiting ROS and cytochrome C.; Authors - Yang Che
mouse	WB	J Alzheimers Dis; Regulation of the Human IL-10RB Gene Expression by Sp8 and Sp9; Authors - Shuyue Luo
mouse	WB	PLoS One; Endogenous interleukin-22 prevents cardiac rupture after myocardial infarction in mice; Authors - Mai Yamamoto