Anticorps Monoclonal anti-IL-12RB1

• Phare
• Validé par KD/KO

IL-12RB1 Monoclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

1E10E10

N° de cat : 66829-1-Ig

Synonymes

CD212, IL 12 receptor beta component, IL 12 receptor subunit beta 1, IL 12R beta 1, IL 12R BETA1, IL 12R subunit beta 1, IL 12RB1, IL12R, IL12RB, IL12RB1, IL-12RB1

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Galerie de données de validation

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  WB analysis of Ramos using 66829-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66829-1-Ig (IL12RB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66829-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66829-1-Ig (IL12RB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66829-1-Ig

  WB result of IL-12RB1 antibody (66829-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-IL-12RB1 transfected Jurkat cells.

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  WB analysis of THP-1 using 66829-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66829-1-Ig (IL12RB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66829-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66829-1-Ig (IL12RB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 66829-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66829-1-Ig (IL12RB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using 66829-1-Ig

  1X10^6 K-562 cells were stained with 0.20 ug/test Anti-Human IL12RB1 (66829-1-Ig, Clone:1E10E10) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules Ramos, cellules Daudi, cellules Jurkat, cellules K-562, cellules Raji, cellules TF-1, cellules THP-1
• Résultats positifs en cytométrie: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66829-1-Ig cible IL-12RB1 dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-12RB1 Protéine recombinante Ag6920
• Nom complet: interleukin 12 receptor, beta 1
• Masse moléculaire calculée: 662 aa, 73 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC029121
• Symbole du gène: IL12RB1
• Identification du gène (NCBI): 3594
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL12RB1, also named as Interleukin-12 receptor subunit beta-1 or CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. Type 2 subfamily. IL12RB1 localizes in the membrane and functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.