Anticorps Polyclonal de lapin anti-IL-17RB

IL-17RB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 20673-1-AP

Synonymes

IL17RB, IL-17 receptor homolog 1, IL 17Rh1, IL 17RB, EVI27

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Galerie de données de validation

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  WB analysis using 20673-1-AP

  Human testis tissue was subjected to SDS PAGE followed by western blot with 20673-1-AP (IL-17RB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20673-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20673-1-AP (IL-17RB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 20673-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20673-1-AP (IL17RB antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 20673-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20673-1-AP (IL17RB antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 20673-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20673-1-AP (IL-17RB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 20673-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20673-1-AP (IL-17RB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20673-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20673-1-AP (IL17RB antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 20673-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20673-1-AP (IL17RB antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 20673-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IL-17RB antibody (20673-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, cellules K-562
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du sein humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• ELISA: See 1 publications below

Informations sur le produit

20673-1-AP cible IL-17RB dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: interleukin 17 receptor B
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 56-60 kDa
• Numéro d’acquisition GenBank: NM_018725
• Symbole du gène: IL-17RB
• Identification du gène (NCBI): 55540
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL17RB, also named as CRL4, EVI27 and IL17BR, is a receptor for the proinflammatory cytokines IL17B and IL17E. IL17RB may play a role in controlling the growth and/or differentiation of hematopoietic cells. IL17RB has two isoforms with MW 56 kDa (isoform 1) and 32 kDa (isoform 2). The antibody is specific to IL17RB.

Publications

Species	Application	Title
human	WB	Nat Commun; Induction of IL-25 secretion from tumour-associated fibroblasts suppresses mammary tumour metastasis.; Authors - Shu-Yi Yin
human	WB,IF	Cell Rep; Schwann cell promotes macrophage recruitment through IL-17B/IL-17RB pathway in injured peripheral nerves; Authors - Yanju Huang
human	IF	Respir Res; IL-25 induces airway remodeling in asthma by orchestrating the phenotypic changes of epithelial cell and fibrocyte; Authors - Xiujuan Yao
human	WB,IF	Front Immunol; Interleukin-25-Mediated-IL-17RB Upregulation Promotes Cutaneous Wound Healing in Diabetic Mice by Improving Endothelial Cell Functions.; Authors - Fang Zhang
	WB	Arch Toxicol; Fluoride induces apoptosis and autophagy through the IL-17 signaling pathway in mice hepatocytes.; Authors - Yangfei Zhao
human	WB	mBio; Viral Mimicry of Interleukin-17A by SARS-CoV-2 ORF8.; Authors - Xin Wu

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