Anticorps Monoclonal anti-IL-18

• Phare
• Validé par KD/KO

IL-18 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

26

CloneNo.

5C6F8

Avis (1)

N° de cat : 60070-1-Ig

Synonymes

Iboctadekin, IFN gamma inducing factor, IGIF, IL 1 gamma, IL 18, IL 1g, IL18, IL-18, IL1F4, Interleukin 1 gamma, Interleukin 18, pre-IL18

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Galerie de données de validation

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  WB analysis using 60070-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60070-1-Ig (IL18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60070-1-Ig

  WB result of Pre-IL18 antibody (60070-1-Ig, 1:4000) with si-Control and si-Pre-IL18 transfected HeLa cells..

• 

  WB analysis using 60070-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60070-1-Ig (IL-18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 60070-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60070-1-Ig (IL18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 60070-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60070-1-Ig (IL18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 60070-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60070-1-Ig (IL-18 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 60070-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60070-1-Ig (IL-18 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 60070-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-18 antibody (60070-1-Ig, Clone: 5C6F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules A549, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'amygdalite humain,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 22 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Informations sur le produit

60070-1-Ig cible IL-18 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-18 Protéine recombinante Ag1063
• Nom complet: IL-18
• Masse moléculaire calculée: 193 aa, 22 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC007461
• Symbole du gène: IL18
• Identification du gène (NCBI): 3606
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 24 kDa precursor and then cleaved into a biologically activate 18 kDa form.

Publications

Species	Application	Title
human	WB	Nature; Recognition and maturation of IL-18 by caspase-4 noncanonical inflammasome; Authors - Xuyan Shi
	WB	J Neuroinflammation; GPR120 modulates epileptic seizure and neuroinflammation mediated by NLRP3 inflammasome.; Authors - Zhangjin Qin
rat	WB	J Ethnopharmacol; Neuroprotective effects of Jie-du-huo-xue decoction on microglia pyroptosis after cerebral ischemia and Reperfusion--From the perspective of glial-vascular unit; Authors - Chang Zhou
human	IHC, WB	Int J Biol Sci; Cisplatin Induces Pyroptosis via Activation of MEG3/NLRP3/caspase-1/GSDMD Pathway in Triple-Negative Breast Cancer.; Authors - Honglin Yan
	WB	Front Microbiol; Methamphetamine Disturbs Gut Homeostasis and Reshapes Serum Metabolome, Inducing Neurotoxicity and Abnormal Behaviors in Mice.; Authors - Kai-Kai Zhang
	WB	Liver Transpl; Endogenous Interleukin-18 Suppresses Liver Regeneration after Hepatectomy in Mice.; Authors - Tao Ma

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Bethany (Verified Customer) (01-05-2022)

Didn't work.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse kidney tissue