Anticorps Monoclonal anti-IL-18

• Phare
• Validé par KD/KO

IL-18 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(45)

CloneNo.

5C6F8

Avis (1)

N° de cat : 60070-1-Ig

Synonymes

IL18, Pre-IL18, 5C6F8, Iboctadekin, IFN-gamma-inducing factor

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Galerie de données de validation

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  WB analysis using 60070-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60070-1-Ig (IL18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60070-1-Ig

  WB result of IL-18 antibody (60070-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IL-18 transfected HeLa cells.

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  WB analysis of HeLa using 60070-1-Ig

  WB result of Pre-IL18 antibody (60070-1-Ig, 1:4000) with si-Control and si-Pre-IL18 transfected HeLa cells..

• 

  WB analysis using 60070-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60070-1-Ig (IL-18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 60070-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60070-1-Ig (IL18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60070-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60070-1-Ig (IL18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 60070-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60070-1-Ig (IL-18 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 60070-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60070-1-Ig (IL-18 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 60070-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-18 antibody (60070-1-Ig, Clone: 5C6F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 60070-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IL-18 antibody (60070-1-Ig, Clone: 5C6F8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  FC experiment of HeLa using 60070-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug IL-18 Monoclonal antibody (60070-1-Ig, Clone:5C6F8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 60070-1-Ig

  Biolayer interferometry (BLl) kinetic assays of 60070-1-Ig against Human Pre-IL18 were performed. The Affinity Constant is 2.79 nM.

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules A549, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 38 publications below
• IHC: See 6 publications below
• IF: See 8 publications below
• ELISA: See 1 publications below

Informations sur le produit

60070-1-Ig cible IL-18 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-18 Protéine recombinante Ag1063
• Nom complet: IL-18
• Masse moléculaire calculée: 193 aa, 22 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC007461
• Symbole du gène: IL-18
• Identification du gène (NCBI): 3606
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL-18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 24 kDa precursor and then cleaved into a biologically activate 18 kDa form.

Publications

Species	Application	Title
human	WB	Nature; Recognition and maturation of IL-18 by caspase-4 noncanonical inflammasome; Authors - Xuyan Shi
	WB	Stem Cells Transl Med; Mesenchymal Stem Cells-Derived Exosomes Alleviate Acute Lung Injury By Inhibiting Alveolar Macrophage Pyroptosis; Authors - Peipei Liu
	WB	J Neuroinflammation; GPR120 modulates epileptic seizure and neuroinflammation mediated by NLRP3 inflammasome.; Authors - Zhangjin Qin
	WB	Int Immunopharmacol; Long non-coding RNA NEAT1 exacerbates NLRP3-mediated pyroptosis in allergic rhinitis through regulating the PTBP1/FOXP1 cascade; Authors - Yunliang Liu
rat	WB	J Ethnopharmacol; Neuroprotective effects of Jie-du-huo-xue decoction on microglia pyroptosis after cerebral ischemia and Reperfusion--From the perspective of glial-vascular unit; Authors - Chang Zhou
rat	WB	J Inflamm (Lond); Carvacrol alleviates LPS-induced myocardial dysfunction by inhibiting the TLR4/MyD88/NF-κB and NLRP3 inflammasome in cardiomyocytes; Authors - Lu Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Bethany (Verified Customer) (01-05-2022)

Didn't work.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse kidney tissue

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