Anticorps Polyclonal de lapin anti-IL-1 alpha

IL-1 alpha Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(47)

N° de cat : 16765-1-AP

Synonymes

IL 1 alpha, IL1A, Hematopoietin-1, IL 1A, IL1 ALPHA

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Galerie de données de validation

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  WB analysis using 16765-1-AP

  Untreated THP-1 cells, THP-1 cells (treated with 50 nM PMA and 5 ug/ml LPS for 24 hours, with addition of 300ng/ml Brefeldin A for the last 3 hours.) were subjected to SDS PAGE followed by western blot with 16765-1-AP (IL-1 alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human appendicitis using 16765-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16765-1-AP (IL-1 alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 16765-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16765-1-AP (IL-1 alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: PMA, LPS and Brefeldin A treated THP-1 cells,
• Résultats positifs en IHC: tissu d'appendicite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 26 publications below
• IHC: See 15 publications below
• IF: See 11 publications below
• ELISA: See 1 publications below

Informations sur le produit

16765-1-AP cible IL-1 alpha dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-1 alpha Protéine recombinante Ag10339
• Nom complet: interleukin 1, alpha
• Masse moléculaire calculée: 271 aa, 31 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC013142
• Symbole du gène: IL-1 alpha
• Identification du gène (NCBI): 3552
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-1α (IL-1 alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 Alpha is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1 Alpha also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. IL-1 Alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 Alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth. IL-1 Alpha is initially translated as approximately 30-kD polypeptides that are processed to approximately 17.5-kD polypeptides prior to, or during, release from macrophages.

Publications

Species	Application	Title
human	WB	Cell Metab; Lighting up arginine metabolism reveals its functional diversity in physiology and pathology; Authors - Rui Li
mouse	IHC	Nat Commun; A stress-induced cilium-to-PML-NB route drives senescence initiation; Authors - Xiaoyu Ma
human	IF	Nat Commun; Schwann cells regulate tumor cells and cancer-associated fibroblasts in the pancreatic ductal adenocarcinoma microenvironment; Authors - Meilin Xue
mouse	WB	Brain Behav Immun; Egln3 expression in microglia enhances the neuroinflammatory responses in Alzheimer's disease; Authors - Jiaxin Guan
human	WB	Gut Microbes; Fusobacterium nucleatum promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway; Authors - Jian-Wei Zhang
		Cancer Lett; Pharmacological inhibition of KDM1A/LSD1 enhances estrogen receptor beta-mediated tumor suppression in ovarian cancer; Authors - Prabhakar Pitta Venkata

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