Anticorps Monoclonal anti-IL-1R2

• Phare
• Validé par KD/KO

IL-1R2 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

3H4H4

N° de cat : 60262-1-Ig

Synonymes

IL1R2, 3H4H4, CD121 antigen-like family member B, CD121b, IL 1R 2

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Galerie de données de validation

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  WB analysis of HeLa using 60262-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60262-1-Ig (IL1R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 60262-1-Ig

  Immunofluorescent analysis of HeLa cells using 60262-1-Ig (IL1R2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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  IF Staining of Jurkat using 60262-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using IL-1R2 antibody (60262-1-Ig, Clone: 3H4H4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: cellules HeLa
• Résultats positifs en IF/ICC: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Informations sur le produit

60262-1-Ig cible IL-1R2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IL-1R2 Protéine recombinante Ag4495
• Nom complet: interleukin 1 receptor, type II
• Masse moléculaire calculée: 398 aa, 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC039031
• Symbole du gène: IL1R2
• Identification du gène (NCBI): 7850
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-1 receptor type 2 (IL1R2, also known as CD121b) is a cytokine receptor that belongs to the interleukin 1 receptor family. It exists in both a membrane bound and soluble form. IL1R2 binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. IL1R2 serves as a negative regulator of IL-1 signaling, thereby suppressing inflammatory response.

Publications

Species	Application	Title
rat	WB	Mater Today Bio; Intracellular hydrogelation of macrophage conjugated probiotics for hitchhiking delivery and combined treatment of colitis; Authors - Jingzhe Wang
mouse	WB	Comput Struct Biotechnol J; Identification of potential diagnostic and prognostic biomarkers for sepsis based on machine learning; Authors - Li Ke
human	WB	J Pancreatol; Immune-related signature identifies IL1R2 as an immunological and prognostic biomarker in pancreatic cancer; Authors - Chengcheng Wang ; KD Validated
mouse	WB,IF	Biochem Pharmacol; Oridonin employs interleukin 1 receptor type 2 to treat noise-induced hearing loss by blocking inner ear inflammation; Authors - Meng-Bing Chen
mouse	WB	Biomacromolecules; Biomimetic NO Scavenging Hyaluronic Acid Nanoparticles Enable Targeted Delivery of MTX and Integrated Management of Rheumatoid Arthritis; Authors - Yilin Liu
	WB	Nat Commun; Targeted therapies of inflammatory diseases with intracellularly gelated macrophages in mice and rats; Authors - Cheng Gao

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