Anticorps Polyclonal de lapin anti-IL-1RA

IL-1RA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 10844-1-AP

Synonymes

IL 1ra, IL1RN, ICIL-1RA, IL 1RN, IL1RA

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Galerie de données de validation

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  WB analysis using 10844-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10844-1-AP (IL-1RA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10844-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10844-1-AP (IL-1RA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human oesophagus using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10844-1-AP (IL-1RA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10844-1-AP (IL1RA antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 10844-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 10844-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using IL-1RA antibody (10844-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of A431 using 10844-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human IL-1RA (10844-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules MOLT-4, cellules RAW 264.7
• Résultats positifs en IHC: tissu œsophagien humain, tissu de cancer du côlon humain, tissu de côlon humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,
• Résultats positifs en FC (Intra): cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10844-1-AP cible IL-1RA dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-1RA Protéine recombinante Ag1277
• Nom complet: interleukin 1 receptor antagonist
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 18-20 kDa
• Numéro d’acquisition GenBank: BC009745
• Symbole du gène: IL-1RA
• Identification du gène (NCBI): 3557
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-1 receptor antagonist (IL-1RA) is a critical member of the IL-1 family of cytokines, which plays a significant role in modulating inflammatory responses. IL-1RA exists in two main forms: a secreted form (sIL-1Ra) and an intracellular form (icIL-1Ra). The secreted form is produced by various cells including monocytes, macrophages, neutrophils, and other cells, while the intracellular form is found in keratinocytes and other epithelial cells, as well as in monocytes and fibroblasts. IL-1RA is important in host defense against endotoxin-induced injury and is produced in numerous experimental animal models of disease, as well as in human autoimmune and chronic inflammatory diseases. It acts as an anti-inflammatory agent by maintaining the balance between pro-inflammatory IL-1α/β and its own anti-inflammatory effects.

Publications

Species	Application	Title
human	WB	Clin Transl Oncol; Inhibitory effect and mechanism of mesenchymal stem cells on melanoma cells.; Authors - J Zhang
human	WB	NeuroImmune Pharm Ther; Resveratrol and its analogs suppress HIV replication, oxidative stress, and inflammation in macrophages; Authors - Santosh Kumar
human	IHC	Breast Cancer (Dove Med Press); Identification and Validation of Novel Metastasis-Related Immune Gene Signature in Breast Cancer; Authors - Shen Ma
mouse	IF	Front Immunol; The influence of angiopoietin-like protein 3 on macrophages polarization and its effect on the podocyte EMT in diabetic nephropathy; Authors - Yanli Ma

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sunniva (Verified Customer) (01-03-2025)

This antibody was able to identify our desired target expressions in Flow Cytometry.

• Applications: Flow Cytometry
• Cell Tissue Type: B cell

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