Anticorps Polyclonal de lapin anti-IL-20RB

IL-20RB Polyclonal Antibody for FC, IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 20521-1-AP

Synonymes

DIRS1, FNDC6, IL 20 receptor subunit beta, IL 20R beta, IL 20R2, IL 20RB, IL20RB, IL-20RB, interleukin 20 receptor beta

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Galerie de données de validation

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  WB analysis of MCF-7 using 20521-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20521-1-AP (IL20RB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20521-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20521-1-AP (IL20RB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20521-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20521-1-AP (IL20RB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Apoptosised HeLa using 20521-1-AP

  Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 20521-1-AP (IL20RB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20521-1-AP

  Immunofluorescent analysis of HepG2 cells, using IL20RB antibody 20521-1-AP at 1:25 dilution and FITC- labeled goat anti-Rabbit IgG (green).

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  FC experiment of K-562 using 20521-1-AP

  1X10^6 K-562 cells were stained with 0.2ug IL20RB antibody (20521-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules MCF7, cellules HeLa, cellules HeLa apoptosées, cellules K-562
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

20521-1-AP cible IL-20RB dans les applications de WB, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-20RB Protéine recombinante Ag14368
• Nom complet: interleukin 20 receptor beta
• Masse moléculaire calculée: 311 aa, 35 kDa
• Poids moléculaire observé: 45 kDa, 66-70 kDa
• Numéro d’acquisition GenBank: BC033292
• Symbole du gène: IL20RB
• Identification du gène (NCBI): 53833
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-20 receptor subunit beta (IL20RB) is a single-pass type I membrane protein of the type II cytokine receptor family. The interleukin-20-receptor I complex (IL-20-RI) is composed of two chains, IL20RA and IL20RB. IL-20-RI is a receptor for IL-19, IL-20 and IL-24. IL20RB can also form a heterodimer with IL22RA1. The IL22RA1/IL20RB dimer is a receptor for IL20 and IL24.

Publications

Species	Application	Title
human	FC	Front Immunol; Integrative single-cell transcriptomic investigation unveils long non-coding RNAs associated with localized cellular inflammation in psoriasis; Authors - Yuge Gao
human,mouse	WB,IF	J Transl Med; IL20RB signaling enhances stemness and chemotherapy resistance in pancreatic cancer; Authors - Xiao-Hui Li