Anticorps Polyclonal de lapin anti-IL-21R

IL-21R Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 10533-1-AP

Synonymes

CD360, IL 21 receptor, IL 21R, IL21R, IL-21R, interleukin 21 receptor, NILR, Novel interleukin receptor

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Galerie de données de validation

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  WB analysis using 10533-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10533-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 10533-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10533-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 10533-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10533-1-AP (IL21R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 10533-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10533-1-AP (IL21R Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 10533-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10533-1-AP (IL21R Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of COLO 320 using 10533-1-AP

  Immunofluorescent analysis of COLO 320 cells using 10533-1-AP (IL21R antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of K-562 using 10533-1-AP

  1X10^6 K-562 cells were stained with 0.2ug IL21R antibody (10533-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules K-562, cellules Raji, tissu de thymus de rat, tissu de thymus de souris
• Résultats positifs en IHC: tissu de lymphome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules COLO 320,
• Résultats positifs en cytométrie: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

10533-1-AP cible IL-21R dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-21R Protéine recombinante Ag0762
• Nom complet: interleukin 21 receptor
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 68-70 kDa
• Numéro d’acquisition GenBank: BC004348
• Symbole du gène: IL21R
• Identification du gène (NCBI): 50615
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL21R, a receptor for interleukin-21, belongs to the type I cytokine receptor family. IL21R has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. This receptor transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3.

Publications

Species	Application	Title
human	IHC	J Immunother Cancer; Prognostic value of novel immune-related genomic biomarkers identified in head and neck squamous cell carcinoma.; Authors - Yao Yao
human	WB,IF	Stem Cells Int; Interleukin 21 Receptor Affects Adipogenesis of Human Adipose-Derived Stem/Stromal Cells.; Authors - Bruna Cristina Falavinha
rat	WB,IF	Int Immunopharmacol; Role of Interleukin-21 in retinal ischemia-reperfusion injury: Unveiling the impact on retinal ganglion cell apoptosis; Authors - Xue Zhang