Anticorps Polyclonal de lapin anti-IL-22RA2

IL-22RA2 Polyclonal Antibody for FC, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, FC,ELISA

Conjugaison

Non conjugué

N° de cat : 22861-1-AP

Synonymes

CRF2 10, CRF2 S1, CRF2X, IL 22 receptor subunit alpha 2, IL 22BP, IL 22R alpha 2, IL 22RA2, IL22BP, IL22RA2, IL-22RA2, Interleukin 22 binding protein, ZcytoR16

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Galerie de données de validation

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  WB analysis of MDA-MB-453s using 22861-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 22861-1-AP (IL22RA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 22861-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22861-1-AP (IL22RA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 22861-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22861-1-AP (IL22RA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22861-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22861-1-AP (IL22RA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22861-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22861-1-AP (IL22RA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 22861-1-AP

  1X10^6 HeLa cells were stained with 0.2ug IL22RA2 antibody (22861-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-453s
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

22861-1-AP cible IL-22RA2 dans les applications de WB, IHC, FC,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-22RA2 Protéine recombinante Ag18854
• Nom complet: interleukin 22 receptor, alpha 2
• Masse moléculaire calculée: 263 aa, 31 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC125167
• Symbole du gène: IL22RA2
• Identification du gène (NCBI): 116379
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-22 receptor subunit alpha-2 (IL22RA2, also known as CRF2-10) is a soluble member of the type II cytokine receptor family. Through binding to IL22, IL22RA2 prevents the interaction of IL22 with the functional IL22-R complex and blocks the activity of IL22 (in vitro). IL22RA2 may play an important role as an IL22 antagonist in the regulation of inflammatory responses. It has been described that human IL22RA2 gene encodes three alternatively spliced isoforms called CRF2-10L, CRF2-10, and CRF2-10s, with molecular weights of 31 kDa, 27 kDa, and 15 kDa, respectively.

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