Anticorps Polyclonal de lapin anti-IL-24

• Phare
• Validé par KD/KO

IL-24 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 26772-1-AP

Synonymes

IL24, Melanoma differentiation-associated gene 7 protein, Interleukin-24, interleukin 24, IL 24

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Galerie de données de validation

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  WB analysis using 26772-1-AP

  Mouse spleen tissues were subjected to SDS PAGE followed by western blot with 26772-1-AP (IL-24 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 26772-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 26772-1-AP (IL-24 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 26772-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 26772-1-AP (IL-24 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 26772-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 26772-1-AP (IL24 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human malignant melanoma using 26772-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 26772-1-AP (IL24 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu splénique de souris,
• Résultats positifs en IHC: tissu de côlon humain, tissu de mélanome malin humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

26772-1-AP cible IL-24 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-24 Protéine recombinante Ag25071
• Nom complet: interleukin 24
• Masse moléculaire calculée: 207 aa, 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC009681
• Symbole du gène: IL-24
• Identification du gène (NCBI): 11009
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL24, also named as MDA-7, MDA7 or ST16, belongs to the IL-10 family. It has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation. The induction of IL24 by oncogenes may support tumor growth at the early stages of cancer. The observed molecular weight of IL24 is reported between 25 and 35 kDa, a value higher than the deduced molecular weight of 23 kDa. This difference is likely due to post-translational modifications, such as glycosylation of the endogenously produced molecule.

Publications

Species	Application	Title
mouse	IHC,IF	Elife; A systematic CRISPR screen reveals an IL-20/IL20RA-mediated immune crosstalk to prevent the ovarian cancer metastasis.; Authors - Jia Li
human	WB	Neoplasia; ZBTB7A Enhances Osteosarcoma Chemoresistance by Transcriptionally Repressing lncRNALINC00473-IL24 Activity.; Authors - Lu Zhang
human	WB	Acta Pharmacol Sin; Targeting PRSS23 with tipranavir induces gastric cancer stem cell apoptosis and inhibits growth of gastric cancer via the MKK3/p38 MAPK-IL24 pathway; Authors - Ji-Xian Xiong ; KD Validated
human	WB	Cell Biol Int; Serum IL-24 combined with CA125 as screening and prognostic biomarkers for NSCLC; Authors - Kai Zhang
human	IF	Exp Mol Med; Single-cell RNA sequencing and spatial transcriptomics of esophageal squamous cell carcinoma with lymph node metastases; Authors - Wei Guo

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