Anticorps Polyclonal de lapin anti-IL-32

• Phare
• Validé par KD/KO

IL-32 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 11079-1-AP

Synonymes

IL 32, IL 32alpha, IL 32beta, IL 32delta, IL 32gamma, IL32, IL-32, interleukin 32, Natural killer cells protein 4, NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd

---

Galerie de données de validation

• 

  IHC staining of human lung cancer using 11079-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11079-1-AP (IL-32 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11079-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11079-1-AP (IL-32 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11079-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11079-1-AP (IL-32 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11079-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11079-1-AP (IL-32 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 6 publications below
• IF: See 2 publications below
• ELISA: See 1 publications below

Informations sur le produit

11079-1-AP cible IL-32 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-32 Protéine recombinante Ag1484
• Nom complet: interleukin 32
• Masse moléculaire calculée: 27 kDa
• Numéro d’acquisition GenBank: BC009401
• Symbole du gène: IL-32
• Identification du gène (NCBI): 9235
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin 32 (IL32) is a member of the cytokine family. IL32 contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. IL32 is a predominantly intracellular proinflammatory cytokine produced by epithelial cells, monocytes, T-lymphocytes and natural killer cells, and is involved in inflammation and cancer development. IL32 is able to induce the release a variety of pro-inflammatory cytokines such as IL8, tumor necrosis factor-α (TNFα) and IL6. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2.

Publications

Species	Application	Title
human	WB	J Cell Mol Med; MicroRNA-29b-3p suppresses oral squamous cell carcinoma cell migration and invasion via IL32/AKT signalling pathway.; Authors - Jianya He
human	IHC	J Hepatocell Carcinoma; Identification and Validation of Novel Biomarkers for Hepatocellular Carcinoma, Liver Fibrosis/Cirrhosis and Chronic Hepatitis B via Transcriptome Sequencing Technology.; Authors - Dandan Zhao
human,mouse	IHC,IF	Front Pharmacol; Targeting TIGIT Inhibits Bladder Cancer Metastasis Through Suppressing IL-32.; Authors - Kang Wu
human	WB,IHC	Oncol Lett; Interleukin-32 regulates downstream molecules and promotes the invasion of pancreatic cancer cells.; Authors - Kohji Takagi ; KD Validated
human	WB,IF	Oncol Lett; Establishment of highly invasive pancreatic cancer cell lines and the expression of IL-32.; Authors - Kohji Takagi
human	WB	Biomed Res Int; IL-32 Promotes the Radiosensitivity of Esophageal Squamous Cell Carcinoma Cell through STAT3 Pathway.; Authors - Zhiyu Ma

{{ptg:RelatedPrimaryAntibodies}}