Anticorps Polyclonal de lapin anti-CXCR2

• Phare
• Validé par KD/KO

CXCR2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 20634-1-AP

Synonymes

CD182, CDw128b, CMKAR2, CXC R2, CXCR 2, CXCR2, GRO/MGSA receptor, IL 8 receptor type 2, IL 8R B, IL8R2, IL8RA, IL8RB, IL-8RB, interleukin 8 receptor, beta

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Galerie de données de validation

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  WB analysis of L02 using 20634-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20634-1-AP (CXCR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 20634-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20634-1-AP (CXCR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 20634-1-AP

  IP Result of anti-CXCR2 (IP:20634-1-AP, 4ug; Detection:20634-1-AP 1:1000) with HepG2 cells lysate 3200ug.

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  IHC staining of human tonsillitis using 20634-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20634-1-AP (CXCR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 20634-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20634-1-AP (CXCR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 20634-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20634-1-AP (CXCR2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 20634-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20634-1-AP (CXCR2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 20634-1-AP

  Immunofluorescent analysis of HepG2 cells, using IL8RB antibody 20634-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules COLO 320
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 4 publications below
• IF: See 9 publications below
• CoIP: See 1 publications below

Informations sur le produit

20634-1-AP cible CXCR2 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CXCR2 Protéine recombinante Ag14670
• Nom complet: interleukin 8 receptor, beta
• Masse moléculaire calculée: 360 aa, 41 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC037961
• Symbole du gène: IL8RB
• Identification du gène (NCBI): 3579
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IL8RB, also named as CXCR2, GRO/MGSA receptor, IL-8 receptor type 2, CDw128b, and CD182, belongs to the G-protein coupled receptor 1 family. It is a receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. The binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. It binds to IL-8 with high affinity. IL8RB also binds with high affinity to CXCL3, GRO/MGSA, and NAP-2.

Publications

Species	Application	Title
human	IF	Cell Rep; Prostate cancer cell-derived exosomal IL-8 fosters immune evasion by disturbing glucolipid metabolism of CD8+ T cell; Authors - Fan Xu
human	WB	Sci Signal; Skewing cPLA2α activity toward oxoeicosanoid production promotes neutrophil N2 polarization, wound healing, and the response to sepsis; Authors - Kenneth D Maus
human	WB	Int J Mol Sci; MyD88 in Macrophages Enhances Liver Fibrosis by Activation of NLRP3 Inflammasome in HSCs.; Authors - Shuang Ge
human	IF	Front Immunol; CXCL5 Downregulation in Villous Tissue Is Correlated With Recurrent Spontaneous Abortion; Authors - Sainan Zhang
human	WB,IHC,IF	Pharmaceuticals (Basel); CXCL8 Promotes Endothelial-to-Mesenchymal Transition of Endothelial Cells and Protects Cells from Erastin-Induced Ferroptosis via CXCR2-Mediated Activation of the NF-κB Signaling Pathway; Authors - Hai-Zhou Ji ; KD Validated
human	IF	JCI Insight; Neutralization of IL-8 decreases tumor PMN-MDSCs and reduces mesenchymalization of claudin-low triple-negative breast cancer.; Authors - Charli Dominguez

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