Anticorps Polyclonal de lapin anti-INF2

• Phare
• Validé par KD/KO

INF2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

28

Avis (2)

N° de cat : 20466-1-AP

Synonymes

C14orf151, C14orf173, HBEBP2 binding protein C, INF2, Inverted formin 2, pp9484

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Galerie de données de validation

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  WB analysis using 20466-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20466-1-AP (INF2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 20466-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20466-1-AP (INF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20466-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20466-1-AP (INF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20466-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20466-1-AP (INF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 20466-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20466-1-AP (INF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 20466-1-AP

  IP Result of anti-INF2 (IP:20466-1-AP, 4ug; Detection:20466-1-AP 1:500) with NIH/3T3 cells lysate 4000ug.

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  IHC staining of human kidney using 20466-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20466-1-AP (INF2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 20466-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20466-1-AP (INF2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of NIH/3T3 using 20466-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 20466-1-AP (INF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A431, cellules HeLa, cellules K-562, cellules NIH/3T3, tissu cérébral de souris
• Résultats positifs en IP: cellules NIH/3T3
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 17 publications below
• CoIP: See 1 publications below

Informations sur le produit

20466-1-AP cible INF2 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: INF2 Protéine recombinante Ag14302
• Nom complet: inverted formin, FH2 and WH2 domain containing
• Masse moléculaire calculée: 1249 aa, 136 kDa
• Poids moléculaire observé: 180-200 kDa
• Numéro d’acquisition GenBank: BC008756
• Symbole du gène: INF2
• Identification du gène (NCBI): 64423
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

INF2 is a member of the formin family of actin-regulating proteins. INF2 is peripherally bound to the cytoplasmic face of the endoplasmic reticulum (ER). Mutations in the formin gene INF2 cause focal segmental glomerulosclerosis. The predicted MW of INF2 is around 136 kDa, while higher molecular mass from 140-200 kDa has been reported in literature (26383224,20493814,19366733).

Publications

Species	Application	Title
mouse	IF	Cell; FMN2 Makes Perinuclear Actin to Protect Nuclei during Confined Migration and Promote Metastasis.; Authors - Colleen T Skau
mouse	IF	Nat Commun; GPCR-induced calcium transients trigger nuclear actin assembly for chromatin dynamics.; Authors - Ying Wang
human	IF	EMBO J; Mechanical strain stimulates COPII-dependent secretory trafficking via Rac1; Authors - Santosh Phuyal
mouse	IF	Proc Natl Acad Sci U S A; Inverted formin 2 in focal adhesions promotes dorsal stress fiber and fibrillar adhesion formation to drive extracellular matrix assembly.; Authors - Colleen T Skau
human	WB, IF	Proc Natl Acad Sci U S A; Mechanical stimulation induces formin-dependent assembly of a perinuclear actin rim.; Authors - Xiaowei Shao ; KD Validated
zebrafish	WB,IF	Curr Biol; Calcium Wave Promotes Cell Extrusion.; Authors - Yasuto Takeuchi ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sammy (Verified Customer) (11-05-2023)

Excellent antibody. Detects a band at the correct MW.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HUVEC

INF2 Antibody Western Blot validation (1:1000 dilution) in HUVEC (Cat no:20466-1-AP)

FH

Chun (Verified Customer) (07-03-2019)

This antibody is good.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2,000
• Cell Tissue Type: HEK293

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