Anticorps Polyclonal de lapin anti-ING2

• Phare
• Validé par KD/KO

ING2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 11560-1-AP

Synonymes

ING1L, ING1Lp, ING2, Inhibitor of growth protein 2, p32, p33ING2

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Galerie de données de validation

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  WB analysis of HEK-293 using 11560-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11560-1-AP (ING2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 11560-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11560-1-AP (ING2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11560-1-AP

  IP Result of anti-ING2 (IP:11560-1-AP, 3ug; Detection:11560-1-AP 1:500) with HEK-293 cells lysate 1000ug.

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  IHC staining of human colon cancer using 11560-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 11560-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 11560-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 11560-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 11560-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 11560-1-AP (ING2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu hépatique humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11560-1-AP cible ING2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ING2 Protéine recombinante Ag2121
• Nom complet: inhibitor of growth family, member 2
• Masse moléculaire calculée: 280 aa, 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC030128
• Symbole du gène: ING2
• Identification du gène (NCBI): 3622
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ING2, also named as ING1L, belongs to the ING family and contains a PHD-type zinc finger. It is induced by the DNA-damaging agent neocarzinostatin. As a candidate tumor suppressor, ING2 seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. It is a component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. Recent finding reported that ING2 regulates muscle differentiation by regulating chormatin remodeling program. Catalog#11560-1-AP is a rabbit polyclonal antibody raised against full length ING2 of human origin.

Publications

Species	Application	Title
human	WB	Mol Cell Proteomics; Human family with sequence similarity 60 member A (FAM60A) protein: a new subunit of the Sin3 deacetylase complex.; Authors - Smith Karen T KT
human	WB	Chem Biol; Deacetylase inhibitors dissociate the histone-targeting ING2 subunit from the Sin3 complex.; Authors - Smith Karen T KT ; KD Validated
human	WB	Cancers (Basel); Antithetic hTERT Regulation by Androgens in Prostate Cancer Cells: hTERT Inhibition Is Mediated by the ING1 and ING2 Tumor Suppressors.; Authors - Sophie Bartsch ; KD Validated
human,mouse	WB,IHC,IF	Front Cell Dev Biol; ING2 Controls Mitochondrial Respiration via Modulating MRPL12 Ubiquitination in Renal Tubular Epithelial Cells.; Authors - Ying Yang ; KD Validated
human	IHC	Cancer Lett; Decreased expression of ING2 gene and its clinicopathological significance in hepatocellular carcinoma.; Authors - Zhang Hua-kun HK
	IP	Biochem J; Histone H3 N-terminal mimicry drives a novel network of methyl-effector interactions.; Authors - Jianji Chen