Anticorps Polyclonal de lapin anti-ING3-specific

ING3-specific Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP,ELISA

Conjugaison

Non conjugué

N° de cat : 16187-1-AP

Synonymes

Eaf4, ING2, ING3, p47ING3

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Galerie de données de validation

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  WB analysis of human kidney using 16187-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16187-1-AP (ING3-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16187-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16187-1-AP (ING3-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16187-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16187-1-AP (ING3-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16187-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16187-1-AP (ING3-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 16187-1-AP

  IP Result of anti-ING3-specific (IP:16187-1-AP, 4ug; Detection:16187-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu rénal humain, tissu cardiaque de souris, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

16187-1-AP cible ING3-specific dans les applications de WB, IP,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: inhibitor of growth family, member 3
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: NM_019071
• Symbole du gène: ING3
• Identification du gène (NCBI): 54556
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Members of inhibitor of growth (ING) family function in inhibiting cell growth and inducing apoptosis. They are sequence homologous proteins. ING3 can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. This antibody is specifically against p47ING3.