Anticorps Polyclonal de lapin anti-INO80

• Phare
• Validé par KD/KO

INO80 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(22)

Avis (1)

N° de cat : 18810-1-AP

Synonymes

INO 80, hINO80, EC:3.6.4.-, DNA helicase-related protein INO80, DNA helicase-related INO80 complex homolog 1

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Galerie de données de validation

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  WB analysis using 18810-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18810-1-AP (INO80 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 18810-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18810-1-AP (INO80 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 18810-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INO80 antibody (18810-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules Jurkat
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 16 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 3 publications below
• ChIP: See 4 publications below

Informations sur le produit

18810-1-AP cible INO80 dans les applications de WB, IHC, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: INO80 homolog (S. cerevisiae)
• Masse moléculaire calculée: 1556 aa, 177 kDa
• Poids moléculaire observé: 178 kDa
• Numéro d’acquisition GenBank: BC146785
• Symbole du gène: INO80
• Identification du gène (NCBI): 54617
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

INO80, also named as INOC1 and KIAA1259, belongs to the SNF2/RAD54 helicase family. The INO80 complex remodels chromatin by shifting nucleosomes. INO80 recruited by YY1 to YY1-activated INO80. In vitro, INO80 has double stranded DNA-dependent ATPase activity

Publications

Species	Application	Title
mouse	WB	Nat Genet; Mammalian SWI/SNF continuously restores local accessibility to chromatin.; Authors - Mario Iurlaro ; KO Validated
mouse	WB,IF,ChIP	Cell Stem Cell; INO80 Facilitates Pluripotency Gene Activation in Embryonic Stem Cell Self-Renewal, Reprogramming, and Blastocyst Development.; Authors - Li Wang ; KD Validated
mouse	WB	Mol Cell; Nascent RNA antagonizes the interaction of a set of regulatory proteins with chromatin.; Authors - Lenka Skalska
mouse	chIP	Mol Cell; Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals.; Authors - Yong Xue ; KD Validated
human	WB	Sci Adv; Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair; Authors - Dinh Duc Nguyen
human	WB,IF	Nat Commun; Resolution of R-loops by INO80 promotes DNA replication and maintains cancer cell proliferation and viability.; Authors - Lisa Prendergast ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

G (Verified Customer) (06-13-2022)

INO80 worked well at 1:5000 in our western blots of nuclear extracts from rodent pancreatic beta cells. 1-2ug of antibody was enough for IP reactions. Seems to work in IHC-paraffin of rodent pancreatic tissue although we are currently optimizing conditions.

• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Mouse pancreatic tissue

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