Anticorps Polyclonal de lapin anti-INPP5B

INPP5B Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 15141-1-AP

Synonymes

5PTase, INPP5B, Phosphoinositide 5 phosphatase

---

Galerie de données de validation

• 

  WB analysis of mouse spleen using 15141-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15141-1-AP (INPP5B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15141-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15141-1-AP (INPP5B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 15141-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15141-1-AP (INPP5B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse pancreas using 15141-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15141-1-AP (INPP5B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 15141-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15141-1-AP (INPP5B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu splénique de souris, cellules HeLa, tissu placentaire humain
• Résultats positifs en IHC: tissu pancréatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

15141-1-AP cible INPP5B dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: INPP5B Protéine recombinante Ag7315
• Nom complet: inositol polyphosphate-5-phosphatase, 75kDa
• Masse moléculaire calculée: 113 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC058932
• Symbole du gène: INPP5B
• Identification du gène (NCBI): 3633
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

INPP5B(Type II inositol 1,4,5-trisphosphate 5-phosphatase) is also named as OCRL2,5PTase, 75 kDa inositol polyphosphate-5-phosphatase and belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family. This protein, originally described in platelets, is one of at least three known enzymes capable of dephosphorylating inositol-1,4,5-trisphosphate (IP3) to inositol-1,4-bisphosphate (IP2)(PMID:8125013). It has 4 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	J Am Soc Nephrol; Effects of Proximal Tubule Shortening on Protein Excretion in a Lowe Syndrome Model.; Authors - Megan L Gliozzi
human	WB	Cancer Cell Int; Decreased INPP5B expression predicts poor prognosis in lung adenocarcinoma.; Authors - Jun Deng
human, mouse	WB,IHC	PLoS One; Compensatory Role of Inositol 5-Phosphatase INPP5B to OCRL in Primary Cilia Formation in Oculocerebrorenal Syndrome of Lowe.; Authors - Luo Na N
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Integrated application of transcriptome and metabolomics reveals potential therapeutic targets for the polarization of atherosclerotic macrophages; Authors - Lin Shen
human	IF	J Biol Chem; Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis; Authors - Robert S Kiss

{{ptg:RelatedPrimaryAntibodies}}